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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F14%3A10173621%21RIV15-MSM-11310___
rdf:type
skos:Concept n17:Vysledek
rdfs:seeAlso
http://link.springer.com/protocol/10.1007%2F978-1-62703-643-6_23
dcterms:description
The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation. The budding yeast (Saccharomyces cerevisiae) can serve as a unique experimental system for functional studies of heterologous genes, allowing not only complementation of readily available yeast mutations but also generation of overexpression phenotypes and in some cases also rescue of such phenotypes. Here we summarize the main considerations that have to be taken into account when using the yeast expression system for investigating the function of plant genes participating in cell morphogenesis; outline the strategies of experiment planning, yeast strain selection (or construction), and expression vector choice; and provide detailed protocols for yeast transformation, transformant selection, and phenotype evaluation.
dcterms:title
Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery
skos:prefLabel
Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery Heterologous expression in budding yeast as a tool for studying the plant cell morphogenesis machinery
skos:notation
RIV/00216208:11310/14:10173621!RIV15-MSM-11310___
n3:aktivita
n11:I n11:Z
n3:aktivity
I, Z(MSM0021620858)
n3:dodaniDat
n16:2015
n3:domaciTvurceVysledku
n14:8483906 n14:6879802
n3:druhVysledku
n7:C
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
19001
n3:idVysledku
RIV/00216208:11310/14:10173621
n3:jazykVysledku
n10:eng
n3:klicovaSlova
screeening; cDNA library; phenotype rescue; complementation; inducible expression system; heterologous gene expression; Saccharomyces cerevisiae
n3:klicoveSlovo
n4:screeening n4:heterologous%20gene%20expression n4:complementation n4:phenotype%20rescue n4:cDNA%20library n4:inducible%20expression%20system n4:Saccharomyces%20cerevisiae
n3:kontrolniKodProRIV
[E261D4E5DF9D]
n3:mistoVydani
New York
n3:nazevEdiceCisloSvazku
Methods in molecular biology, 1080
n3:nazevZdroje
Methods in molecular biology (Clifton, N.J.)
n3:obor
n5:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetStranKnihy
285
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n16:2014
n3:tvurceVysledku
Hála, Michal Cvrčková, Fatima
n3:zamer
n22:MSM0021620858
s:numberOfPages
16
n18:doi
10.1007/978-1-62703-643-6_23
n13:hasPublisher
Springer-Verlag
n9:isbn
978-1-62703-643-6
n8:organizacniJednotka
11310