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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10196817%21RIV14-GA0-11310___
rdf:type
skos:Concept n18:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1007/s12224-013-9151-7
dcterms:description
There are only a few exceptions to the rule that polyploidy in Taraxacum is associated with agamospermy. One of them is the sexual, tetraploid species Taraxacum stenocephalum. Incidentally, remarkable variation in karyology was found in this species. The present study aims to confirm this variation by an extensive screen of nuclear DNA content. Individuals from two large populations in the Lesser and Greater Caucasus, Georgia were analyzed using flow cytometry to ascertain intraspecific nuclear DNA content variation. Across the whole data set comprising all 159 individuals, a 1.223-fold difference was detected based on propidium iodide (PI) analyses. To verify this finding, we compared flow-cytometric data obtained using DAPI (4',6-diamidino-2-phenylindole) and PI staining using a representative subset of individuals. This comparison revealed a 1.194-fold difference in DNA content for DAPI and a 1.219-fold difference for PI. Mean nuclear genome size in absolute terms (2C value +/- s.d.) was estimated at 4.38 +/- 0.21 pg, ranging from 4.01 pg to 4.89 pg, despite the invariable chromosome counts of 2n = 32. A regression analysis comparing the datasets for DAPI and PI staining found a strong correlation between data obtained by the DAPI and PI dyes (R = 0.976; P = 0.0001). Simultaneous high-resolution flow-cytometric analyses also proved the accuracy of our findings. We discuss possible sources of these large differences in DNA content within Taraxacum stenocephalum. Further research is needed to identify the source of this remarkable variation. There are only a few exceptions to the rule that polyploidy in Taraxacum is associated with agamospermy. One of them is the sexual, tetraploid species Taraxacum stenocephalum. Incidentally, remarkable variation in karyology was found in this species. The present study aims to confirm this variation by an extensive screen of nuclear DNA content. Individuals from two large populations in the Lesser and Greater Caucasus, Georgia were analyzed using flow cytometry to ascertain intraspecific nuclear DNA content variation. Across the whole data set comprising all 159 individuals, a 1.223-fold difference was detected based on propidium iodide (PI) analyses. To verify this finding, we compared flow-cytometric data obtained using DAPI (4',6-diamidino-2-phenylindole) and PI staining using a representative subset of individuals. This comparison revealed a 1.194-fold difference in DNA content for DAPI and a 1.219-fold difference for PI. Mean nuclear genome size in absolute terms (2C value +/- s.d.) was estimated at 4.38 +/- 0.21 pg, ranging from 4.01 pg to 4.89 pg, despite the invariable chromosome counts of 2n = 32. A regression analysis comparing the datasets for DAPI and PI staining found a strong correlation between data obtained by the DAPI and PI dyes (R = 0.976; P = 0.0001). Simultaneous high-resolution flow-cytometric analyses also proved the accuracy of our findings. We discuss possible sources of these large differences in DNA content within Taraxacum stenocephalum. Further research is needed to identify the source of this remarkable variation.
dcterms:title
Substantial Genome Size Variation in Taraxacum stenocephalum (Asteraceae, Lactuceae) Substantial Genome Size Variation in Taraxacum stenocephalum (Asteraceae, Lactuceae)
skos:prefLabel
Substantial Genome Size Variation in Taraxacum stenocephalum (Asteraceae, Lactuceae) Substantial Genome Size Variation in Taraxacum stenocephalum (Asteraceae, Lactuceae)
skos:notation
RIV/00216208:11310/13:10196817!RIV14-GA0-11310___
n18:predkladatel
n19:orjk%3A11310
n3:aktivita
n16:Z n16:I n16:S n16:P
n3:aktivity
I, P(GA206/05/0970), P(GD206/08/H049), S, Z(AV0Z60050516), Z(MSM0021620828)
n3:cisloPeriodika
2
n3:dodaniDat
n10:2014
n3:domaciTvurceVysledku
n6:1252372 n6:2651416 n6:5025796 n6:3772268
n3:druhVysledku
n22:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
108903
n3:idVysledku
RIV/00216208:11310/13:10196817
n3:jazykVysledku
n21:eng
n3:klicovaSlova
Taraxacum stenocephalum; Intraspecific DNA content variation; Genome size; Flow cytometry
n3:klicoveSlovo
n15:Flow%20cytometry n15:Taraxacum%20stenocephalum n15:Genome%20size n15:Intraspecific%20DNA%20content%20variation
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[0424975624BB]
n3:nazevZdroje
Folia Geobotanica
n3:obor
n9:EF
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n7:GD206%2F08%2FH049 n7:GA206%2F05%2F0970
n3:rokUplatneniVysledku
n10:2013
n3:svazekPeriodika
48
n3:tvurceVysledku
Rooks, Frederick Kirschner, Jan Chudáčková, Hana Trávníček, Pavel Štěpánek, Jan
n3:wos
000320112600008
n3:zamer
n11:AV0Z60050516 n11:MSM0021620828
s:issn
1211-9520
s:numberOfPages
14
n13:doi
10.1007/s12224-013-9151-7
n20:organizacniJednotka
11310