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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10192950%21RIV14-MSM-11310___
rdf:type
skos:Concept n15:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1002/chem.201301868
dcterms:description
Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three-potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications. Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three-potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
dcterms:title
Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases
skos:prefLabel
Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases
skos:notation
RIV/00216208:11310/13:10192950!RIV14-MSM-11310___
n15:predkladatel
n17:orjk%3A11310
n3:aktivita
n18:I n18:P
n3:aktivity
I, P(GBP206/12/G151), P(IAA400040901)
n3:cisloPeriodika
38
n3:dodaniDat
n11:2014
n3:domaciTvurceVysledku
n19:6659160
n3:druhVysledku
n16:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
63106
n3:idVysledku
RIV/00216208:11310/13:10192950
n3:jazykVysledku
n5:eng
n3:klicovaSlova
voltammetry; sequencing; nucleoside triphosphates; electrochemistry; DNA polymerase
n3:klicoveSlovo
n4:sequencing n4:electrochemistry n4:voltammetry n4:DNA%20polymerase n4:nucleoside%20triphosphates
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[B5329F3904CC]
n3:nazevZdroje
Chemistry - A European Journal
n3:obor
n21:CC
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:projekt
n7:IAA400040901 n7:GBP206%2F12%2FG151
n3:rokUplatneniVysledku
n11:2013
n3:svazekPeriodika
19
n3:tvurceVysledku
Balintová, Jana Hocek, Michal Pohl, Radek Fojta, Miroslav Havran, Luděk Plucnara, Medard Vidláková, Pavlína
n3:wos
000324316300025
s:issn
0947-6539
s:numberOfPages
12
n20:doi
10.1002/chem.201301868
n14:organizacniJednotka
11310