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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10191612%21RIV14-MSM-11310___
rdf:type
n14:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.ijbiomac.2013.07.014
dcterms:description
Complementary molecules on the surface of both gametes are responsible for the interaction of sperm protein receptors with zona pellucida (ZP) saccharide structures, and many primary sperm receptors for ZP glycoproteins have been disclosed in various mammals. For our study, proteins were obtained from the surface of ejaculated and in vitro capacitated boar sperm. The isolated proteins were characterized by 1D- and 2D-electrophoretic protein profiles, and by glycoprotein staining. Our results show quantitative and qualitative differences in protein and glycoprotein patterns between ejaculated and capacitated sperm. Far-western blotting with ZP glycoproteins identified 17 interactions in the subproteome of the ejaculated sperm and 14 interactions in the subproteome of the capacitated sperm. High-molecular-mass proteins, coincident with binding to ZP, were sequence-identified. Angiotensin-converting enzyme (ACE), polycystic kidney disease receptor and egg jelly receptor (PKDREJ), and acrosin precursor were successfully identified. This is the first time PKDREJ has been identified on the surface of boar spermatozoa. Complementary molecules on the surface of both gametes are responsible for the interaction of sperm protein receptors with zona pellucida (ZP) saccharide structures, and many primary sperm receptors for ZP glycoproteins have been disclosed in various mammals. For our study, proteins were obtained from the surface of ejaculated and in vitro capacitated boar sperm. The isolated proteins were characterized by 1D- and 2D-electrophoretic protein profiles, and by glycoprotein staining. Our results show quantitative and qualitative differences in protein and glycoprotein patterns between ejaculated and capacitated sperm. Far-western blotting with ZP glycoproteins identified 17 interactions in the subproteome of the ejaculated sperm and 14 interactions in the subproteome of the capacitated sperm. High-molecular-mass proteins, coincident with binding to ZP, were sequence-identified. Angiotensin-converting enzyme (ACE), polycystic kidney disease receptor and egg jelly receptor (PKDREJ), and acrosin precursor were successfully identified. This is the first time PKDREJ has been identified on the surface of boar spermatozoa.
dcterms:title
Characterization of sperm surface protein patterns of ejaculated and capacitated boar sperm, with the detection of ZP binding candidates Characterization of sperm surface protein patterns of ejaculated and capacitated boar sperm, with the detection of ZP binding candidates
skos:prefLabel
Characterization of sperm surface protein patterns of ejaculated and capacitated boar sperm, with the detection of ZP binding candidates Characterization of sperm surface protein patterns of ejaculated and capacitated boar sperm, with the detection of ZP binding candidates
skos:notation
RIV/00216208:11310/13:10191612!RIV14-MSM-11310___
n14:predkladatel
n16:orjk%3A11310
n3:aktivita
n15:Z n15:S n15:P n15:I
n3:aktivity
I, P(GAP503/12/1834), S, Z(AV0Z50520701)
n3:cisloPeriodika
October 2013
n3:dodaniDat
n11:2014
n3:domaciTvurceVysledku
n10:1926144 n10:9501894
n3:druhVysledku
n17:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
65106
n3:idVysledku
RIV/00216208:11310/13:10191612
n3:jazykVysledku
n18:eng
n3:klicovaSlova
PKDREJ protein; Zona pellucida-binding receptors; Sperm surface proteins
n3:klicoveSlovo
n20:Sperm%20surface%20proteins n20:PKDREJ%20protein n20:Zona%20pellucida-binding%20receptors
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[5620A882B930]
n3:nazevZdroje
International Journal of Biological Macromolecules
n3:obor
n21:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n13:GAP503%2F12%2F1834
n3:rokUplatneniVysledku
n11:2013
n3:svazekPeriodika
61
n3:tvurceVysledku
Jonáková, Věra Maňásková-Postlerová, Pavla Zigo, Michal Šulc, Miroslav
n3:wos
000326660700047
n3:zamer
n12:AV0Z50520701
s:issn
0141-8130
s:numberOfPages
7
n19:doi
10.1016/j.ijbiomac.2013.07.014
n22:organizacniJednotka
11310