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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10190914%21RIV14-MSM-11310___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
The injured or otherwise damaged cornea is healed by limbal stem cells (LSC). If the limbus where LSC reside is also damaged or nonfunctional, the cornea cannot heal properly and this defect leads to impaired vision that can result in blindness. The only way to treat total LSC deficiency is by transplantation of limbal tissue or a transfer of LSC. Recently, mesenchymal stem cells (MSC) have been shown as another promising source of stem cells for corneal healing and regeneration. Here, we describe a protocol for the use of polyamide 6/12 nanofiber scaffolds for the growth of MSC and LSC, and for their transfer onto a mechanically damaged ocular surface in the experimental mouse model. The injured or otherwise damaged cornea is healed by limbal stem cells (LSC). If the limbus where LSC reside is also damaged or nonfunctional, the cornea cannot heal properly and this defect leads to impaired vision that can result in blindness. The only way to treat total LSC deficiency is by transplantation of limbal tissue or a transfer of LSC. Recently, mesenchymal stem cells (MSC) have been shown as another promising source of stem cells for corneal healing and regeneration. Here, we describe a protocol for the use of polyamide 6/12 nanofiber scaffolds for the growth of MSC and LSC, and for their transfer onto a mechanically damaged ocular surface in the experimental mouse model.
dcterms:title
The Growth and Delivery of Mezenchymal and Limbal Stem Cells Using Copolymer Polyamide 6/12 Nanofiber Scaffold The Growth and Delivery of Mezenchymal and Limbal Stem Cells Using Copolymer Polyamide 6/12 Nanofiber Scaffold
skos:prefLabel
The Growth and Delivery of Mezenchymal and Limbal Stem Cells Using Copolymer Polyamide 6/12 Nanofiber Scaffold The Growth and Delivery of Mezenchymal and Limbal Stem Cells Using Copolymer Polyamide 6/12 Nanofiber Scaffold
skos:notation
RIV/00216208:11310/13:10190914!RIV14-MSM-11310___
n5:predkladatel
n6:orjk%3A11310
n3:aktivita
n4:S n4:I n4:Z
n3:aktivity
I, S, Z(MSM0021620858)
n3:dodaniDat
n19:2014
n3:domaciTvurceVysledku
n9:5695678 n9:7357354 n9:2767198
n3:druhVysledku
n15:C
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
76919
n3:idVysledku
RIV/00216208:11310/13:10190914
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Ocular surface reconstruction; Polyamide 6/12 nanofiber scaffolds; Limbal stem cells; Mesenchymal stem cells
n3:klicoveSlovo
n17:Limbal%20stem%20cells n17:Mesenchymal%20stem%20cells n17:Ocular%20surface%20reconstruction n17:Polyamide%206%2F12%20nanofiber%20scaffolds
n3:kontrolniKodProRIV
[2DF3BA85A27F]
n3:mistoVydani
New York
n3:nazevEdiceCisloSvazku
Part IV
n3:nazevZdroje
Corneal Regenerative Medicine
n3:obor
n14:EC
n3:pocetDomacichTvurcuVysledku
3
n3:pocetStranKnihy
230
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n19:2013
n3:tvurceVysledku
Holáň, Vladimír Trošan, Peter Javorková, Eliška
n3:zamer
n8:MSM0021620858
s:numberOfPages
13
n18:doi
10.1007/978-1-62703-432-6_13
n13:hasPublisher
Humana Press
n16:isbn
978-1-62703-431-9
n7:organizacniJednotka
11310