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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10190859%21RIV14-MSM-11310___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
32P-Postlabeling analysis is an ultrasensitive method for the detection and quantitation of DNA adducts and covalent modifications of the DNA. It consists of four main steps: (1) enzymatic digestion of DNA to 3'-monophosphate nucleosides; (2) enrichment of the adducts; (3) 5'OH-labeling of the adducts by T4 polynucleotide kinase-catalyzed transfer of 32P-orthophosphate from [γ-32P]ATP; and (4) chromato-graphic or electrophoretic separation of labeled adducts and detection and quantification by means of their radioactive decay. The assay requires only micrograms of DNA and is capable of detecting adduct levels as low as one adduct in 109-1010 normal nucleotides. It is applicable to a wide range of investigations in human, animal, and in vitro studies including monitoring exposure to environmental or occupational carcinogens, determining whether a chemical or a complex mixture has genotoxic properties, elucidation of the toxicological pathways of carcinogens, and monitoring DNA repair. 32P-Postlabeling analysis is an ultrasensitive method for the detection and quantitation of DNA adducts and covalent modifications of the DNA. It consists of four main steps: (1) enzymatic digestion of DNA to 3'-monophosphate nucleosides; (2) enrichment of the adducts; (3) 5'OH-labeling of the adducts by T4 polynucleotide kinase-catalyzed transfer of 32P-orthophosphate from [γ-32P]ATP; and (4) chromato-graphic or electrophoretic separation of labeled adducts and detection and quantification by means of their radioactive decay. The assay requires only micrograms of DNA and is capable of detecting adduct levels as low as one adduct in 109-1010 normal nucleotides. It is applicable to a wide range of investigations in human, animal, and in vitro studies including monitoring exposure to environmental or occupational carcinogens, determining whether a chemical or a complex mixture has genotoxic properties, elucidation of the toxicological pathways of carcinogens, and monitoring DNA repair.
dcterms:title
32P-Postlabeling Analysis of DNA Adducts 32P-Postlabeling Analysis of DNA Adducts
skos:prefLabel
32P-Postlabeling Analysis of DNA Adducts 32P-Postlabeling Analysis of DNA Adducts
skos:notation
RIV/00216208:11310/13:10190859!RIV14-MSM-11310___
n11:predkladatel
n12:orjk%3A11310
n3:aktivita
n19:I
n3:aktivity
I
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n8:8486573
n3:druhVysledku
n9:C
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
119830
n3:idVysledku
RIV/00216208:11310/13:10190859
n3:jazykVysledku
n13:eng
n3:klicovaSlova
thin-layer chromatography; environmental and occupational exposures; DNA damage and repair; genotoxicity testing; human biomonitoring; DNA adducts; 32P-postlabeling
n3:klicoveSlovo
n4:DNA%20adducts n4:genotoxicity%20testing n4:32P-postlabeling n4:human%20biomonitoring n4:thin-layer%20chromatography n4:environmental%20and%20occupational%20exposures n4:DNA%20damage%20and%20repair
n3:kontrolniKodProRIV
[3D23EFEA08F2]
n3:mistoVydani
New York, US
n3:nazevEdiceCisloSvazku
Methods in Molecular Biology, Vol. 1044
n3:nazevZdroje
Genotoxicity Assessment - Methods and Protocols
n3:obor
n15:CE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetStranKnihy
463
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n7:2013
n3:tvurceVysledku
Arlt, Volker M. Stiborová, Marie Schmeiser, Heinz H.
s:numberOfPages
13
n18:doi
10.1007/978-1-62703-529-3_21
n20:hasPublisher
Humana Press Inc.
n14:isbn
978-1-62703-528-6
n21:organizacniJednotka
11310