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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10173228%21RIV14-MSM-11310___
rdf:type
skos:Concept n20:Vysledek
rdfs:seeAlso
http://www.bioone.org/doi/full/10.1525/abt.2013.75.9.13
dcterms:description
To involve students in thinking about the problem of AIDS (which is important in the view of non-decreasing infection rates) we established a practical lab using a simplified method of Thomas (2004) to determine the polymorphism of HIV co-receptor CCR5 from students' own epithelial cells. CCR5 is a receptor involved in inflammatory processes, which has been misused by some pathogens, including HIV, to enter host cells. As a result, a defective allele CCR5-Δ32 has been enriched in some populations. Due to the interesting story and hands-on work with their own tissue students were absorbed by this 2 hour practical. To involve students in thinking about the problem of AIDS (which is important in the view of non-decreasing infection rates) we established a practical lab using a simplified method of Thomas (2004) to determine the polymorphism of HIV co-receptor CCR5 from students' own epithelial cells. CCR5 is a receptor involved in inflammatory processes, which has been misused by some pathogens, including HIV, to enter host cells. As a result, a defective allele CCR5-Δ32 has been enriched in some populations. Due to the interesting story and hands-on work with their own tissue students were absorbed by this 2 hour practical.
dcterms:title
A simplified technique for evaluating human CCR5 genetic polymorphism A simplified technique for evaluating human CCR5 genetic polymorphism
skos:prefLabel
A simplified technique for evaluating human CCR5 genetic polymorphism A simplified technique for evaluating human CCR5 genetic polymorphism
skos:notation
RIV/00216208:11310/13:10173228!RIV14-MSM-11310___
n20:predkladatel
n21:orjk%3A11310
n3:aktivita
n15:Z n15:P n15:I
n3:aktivity
I, P(1M0506), Z(MSM0021620858)
n3:dodaniDat
n10:2014
n3:domaciTvurceVysledku
n14:3443388 n14:8234647 n14:4310314
n3:druhVysledku
n19:O
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
59070
n3:idVysledku
RIV/00216208:11310/13:10173228
n3:jazykVysledku
n18:eng
n3:klicovaSlova
secondary school; polymorphism; genotyping; HIV resistance; CCR5; polymerase chain reaction; PCR
n3:klicoveSlovo
n5:secondary%20school n5:genotyping n5:PCR n5:polymorphism n5:HIV%20resistance n5:polymerase%20chain%20reaction n5:CCR5
n3:kontrolniKodProRIV
[085ACCF7ED0C]
n3:obor
n4:AM
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n13:1M0506
n3:rokUplatneniVysledku
n10:2013
n3:tvurceVysledku
Janštová, Vanda Černý, Jan Falteisek, Lukáš
n3:zamer
n16:MSM0021620858
n8:doi
10.1525/abt.2013.75.9.13
n12:organizacniJednotka
11310