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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10172702%21RIV14-GA0-11310___
rdf:type
n14:Vysledek skos:Concept
rdfs:seeAlso
http://www.bestservis.eu/upload/file/Sbornik_2013.pdf
dcterms:description
In this work, we present several applications that manifest the potential of capillary electrophoresis in enzyme assays. Capillary electrophoresis was employed to perform offline and online assays of beta-N-acetylhexosaminidase for three different substrates. Offline assays were successfully utilized to monitor the enzyme reaction and to determine parameters such as pH and temperature optimum. The offline assay was also used to compare activities of the enzyme towards the natural and chromogenic substrates. Finally, an online enzyme assay was developed and proved to be highly beneficial for inhibitor screening. In this work, we present several applications that manifest the potential of capillary electrophoresis in enzyme assays. Capillary electrophoresis was employed to perform offline and online assays of beta-N-acetylhexosaminidase for three different substrates. Offline assays were successfully utilized to monitor the enzyme reaction and to determine parameters such as pH and temperature optimum. The offline assay was also used to compare activities of the enzyme towards the natural and chromogenic substrates. Finally, an online enzyme assay was developed and proved to be highly beneficial for inhibitor screening.
dcterms:title
Applications of Capillary Electrophoresis in Enzyme Assays Applications of Capillary Electrophoresis in Enzyme Assays
skos:prefLabel
Applications of Capillary Electrophoresis in Enzyme Assays Applications of Capillary Electrophoresis in Enzyme Assays
skos:notation
RIV/00216208:11310/13:10172702!RIV14-GA0-11310___
n14:predkladatel
n17:orjk%3A11310
n3:aktivita
n22:I n22:S n22:P n22:Z
n3:aktivity
I, P(GBP206/12/G151), S, Z(MSM0021620857)
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n9:2245272 n9:2266105 n9:9174400 n9:9608753 n9:6532667
n3:druhVysledku
n13:D
n3:duvernostUdaju
n24:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
61846
n3:idVysledku
RIV/00216208:11310/13:10172702
n3:jazykVysledku
n19:eng
n3:klicovaSlova
electrophoretically mediated microanalysis; chitobiose; chitotriose; beta-N-acetylhexosaminidase; enzyme activity; capillary electrophoresis
n3:klicoveSlovo
n8:electrophoretically%20mediated%20microanalysis n8:beta-N-acetylhexosaminidase n8:enzyme%20activity n8:chitobiose n8:chitotriose n8:capillary%20electrophoresis
n3:kontrolniKodProRIV
[F8504A4C94ED]
n3:mistoKonaniAkce
Jetřichovice, Czech Republic
n3:mistoVydani
Ústí nad Labem
n3:nazevZdroje
Sborník přednášek mezinárodní odborné konference XXXIII. Moderní Elektrochemické Metody Jetřichovice 20. - 24. května 2013
n3:obor
n6:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n20:GBP206%2F12%2FG151
n3:rokUplatneniVysledku
n7:2013
n3:tvurceVysledku
Doubnerová, Veronika Velvarská, Romana Křížek, Tomáš Coufal, Pavel Ryšlavá, Helena
n3:typAkce
n18:EUR
n3:zahajeniAkce
2013-05-20+02:00
n3:zamer
n16:MSM0021620857
s:numberOfPages
5
n12:hasPublisher
Srsenová Lenka - BEST servis Ústí nad Labem
n10:isbn
978-80-905221-1-4
n23:organizacniJednotka
11310