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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10159267%21RIV14-MSM-11310___
rdf:type
n8:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1021/bc400149q
dcterms:description
Enzymatic synthesis of short (10-22 nt) base-modified oligonucleotides (ONs) was developed by nicking enzyme amplification reaction (NEAR) using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease, and a modified deoxyribonucleoside triphosphate (dNTP) derivative. The scope and limitations of the methodology in terms of different nucleobases, length, sequences, and modifications has been thoroughly studied. The methodology including isolation of the modified ONs was scaled up to nanomolar amounts and the modified ONs were successfully used as primers in primer extension and PCR Two simple and efficient methods for fluorescent labeling of the PCR products were developed, based either on direct fluorescent labeling of primers or on NEAR synthesis of ethynylated primers, PCR, and final click labeling with fluorescent azides. Enzymatic synthesis of short (10-22 nt) base-modified oligonucleotides (ONs) was developed by nicking enzyme amplification reaction (NEAR) using Vent(exo-) polymerase, Nt.BstNBI nicking endonuclease, and a modified deoxyribonucleoside triphosphate (dNTP) derivative. The scope and limitations of the methodology in terms of different nucleobases, length, sequences, and modifications has been thoroughly studied. The methodology including isolation of the modified ONs was scaled up to nanomolar amounts and the modified ONs were successfully used as primers in primer extension and PCR Two simple and efficient methods for fluorescent labeling of the PCR products were developed, based either on direct fluorescent labeling of primers or on NEAR synthesis of ethynylated primers, PCR, and final click labeling with fluorescent azides.
dcterms:title
Scope and Limitations of the Nicking Enzyme Amplification Reaction for the Synthesis of Base-Modified Oligonucleotides and Primers for PCR Scope and Limitations of the Nicking Enzyme Amplification Reaction for the Synthesis of Base-Modified Oligonucleotides and Primers for PCR
skos:prefLabel
Scope and Limitations of the Nicking Enzyme Amplification Reaction for the Synthesis of Base-Modified Oligonucleotides and Primers for PCR Scope and Limitations of the Nicking Enzyme Amplification Reaction for the Synthesis of Base-Modified Oligonucleotides and Primers for PCR
skos:notation
RIV/00216208:11310/13:10159267!RIV14-MSM-11310___
n8:predkladatel
n9:orjk%3A11310
n3:aktivita
n11:P n11:I
n3:aktivity
I, P(GA203/09/0317)
n3:cisloPeriodika
6
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n21:6659160
n3:druhVysledku
n13:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
104120
n3:idVysledku
RIV/00216208:11310/13:10159267
n3:jazykVysledku
n4:eng
n3:klicovaSlova
nucleic-acids; protein interactions; restriction endonucleases; high-density; click reaction; functionalized DNA; polymerase incorporation; nucleoside triphosphates; cross-coupling reactions; isothermal DNA amplification
n3:klicoveSlovo
n6:isothermal%20DNA%20amplification n6:high-density n6:nucleoside%20triphosphates n6:polymerase%20incorporation n6:nucleic-acids n6:restriction%20endonucleases n6:protein%20interactions n6:functionalized%20DNA n6:click%20reaction n6:cross-coupling%20reactions
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[D8FAD5F0F7F4]
n3:nazevZdroje
Bioconjugate Chemistry
n3:obor
n14:CC
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
3
n3:projekt
n12:GA203%2F09%2F0317
n3:rokUplatneniVysledku
n7:2013
n3:svazekPeriodika
24
n3:tvurceVysledku
Hocek, Michal Raindlová, Veronika Ménová, Petra
n3:wos
000320898900028
s:issn
1043-1802
s:numberOfPages
13
n20:doi
10.1021/bc400149q
n19:organizacniJednotka
11310