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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10144180%21RIV14-GA0-11310___
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770667/
dcterms:description
The SUN family is comprised of proteins that are conserved among various yeasts and fungi, but that are absent in mammals and plants. Although the function(s) of these proteins are mostly unknown, they have been linked to various, often unrelated cellular processes such as those connected to mitochondrial and cell wall functions. Here we show that three of the four Saccharomyces cerevisiae SUN family proteins, Uth1p, Sim1p and Sun4p, are efficiently secreted out of the cells in different growth phases and their production is affected by the level of oxygen. The Uth1p, Sim1p, Sun4p and Nca3p are mostly synthesized during the growth phase of both yeast liquid cultures and colonies. Culture transition to slowgrowing or stationary phases is linked with a decreased cellular concentration of Sim1p and Sun4p and with their efficient release from the cells. In contrast, Uth1p is released mainly from growing cells. The synthesis of Uth1p and Sim1p, but not of Sun4p, is repressed by anoxia. All four proteins confer cell sensitivity to zymolyase. In addition, Uth1p affects cell sensitivity to compounds influencing cell wall composition and integrity (such as Calcofluor white and Congo red) differently when growing on fermentative versus respiratory carbon sources. In contrast, Uth1p is essential for cell resistance to boric acids irrespective of carbon source. In summary, our novel findings support the hypothesis that SUN family proteins are involved in the remodeling of the yeast cell wall during the various phases of yeast culture development and under various environmental conditions. The finding that Uth1p is involved in cell sensitivity to boric acid, i.e. to a compound that is commonly used as an important antifungal in mycoses, opens up new possibilities of investigating the mechanisms of boric acid's action. The SUN family is comprised of proteins that are conserved among various yeasts and fungi, but that are absent in mammals and plants. Although the function(s) of these proteins are mostly unknown, they have been linked to various, often unrelated cellular processes such as those connected to mitochondrial and cell wall functions. Here we show that three of the four Saccharomyces cerevisiae SUN family proteins, Uth1p, Sim1p and Sun4p, are efficiently secreted out of the cells in different growth phases and their production is affected by the level of oxygen. The Uth1p, Sim1p, Sun4p and Nca3p are mostly synthesized during the growth phase of both yeast liquid cultures and colonies. Culture transition to slowgrowing or stationary phases is linked with a decreased cellular concentration of Sim1p and Sun4p and with their efficient release from the cells. In contrast, Uth1p is released mainly from growing cells. The synthesis of Uth1p and Sim1p, but not of Sun4p, is repressed by anoxia. All four proteins confer cell sensitivity to zymolyase. In addition, Uth1p affects cell sensitivity to compounds influencing cell wall composition and integrity (such as Calcofluor white and Congo red) differently when growing on fermentative versus respiratory carbon sources. In contrast, Uth1p is essential for cell resistance to boric acids irrespective of carbon source. In summary, our novel findings support the hypothesis that SUN family proteins are involved in the remodeling of the yeast cell wall during the various phases of yeast culture development and under various environmental conditions. The finding that Uth1p is involved in cell sensitivity to boric acid, i.e. to a compound that is commonly used as an important antifungal in mycoses, opens up new possibilities of investigating the mechanisms of boric acid's action.
dcterms:title
SUN Family Proteins Sun4p, Uth1p and Sim1p Are Secreted from Saccharomyces cerevisiae and Produced Dependently on Oxygen Level SUN Family Proteins Sun4p, Uth1p and Sim1p Are Secreted from Saccharomyces cerevisiae and Produced Dependently on Oxygen Level
skos:prefLabel
SUN Family Proteins Sun4p, Uth1p and Sim1p Are Secreted from Saccharomyces cerevisiae and Produced Dependently on Oxygen Level SUN Family Proteins Sun4p, Uth1p and Sim1p Are Secreted from Saccharomyces cerevisiae and Produced Dependently on Oxygen Level
skos:notation
RIV/00216208:11310/13:10144180!RIV14-GA0-11310___
n14:predkladatel
n20:orjk%3A11310
n4:aktivita
n9:Z n9:S n9:P n9:I
n4:aktivity
I, P(GA13-08605S), S, Z(MSM0021620858)
n4:cisloPeriodika
9
n4:dodaniDat
n8:2014
n4:domaciTvurceVysledku
n7:1218182 n7:3564053 n7:1621718 n7:1892746
n4:druhVysledku
n19:J
n4:duvernostUdaju
n5:S
n4:entitaPredkladatele
n11:predkladatel
n4:idSjednocenehoVysledku
108992
n4:idVysledku
RIV/00216208:11310/13:10144180
n4:jazykVysledku
n15:eng
n4:klicovaSlova
oscillations; expression; morphogenesis; death; gene; congo red; biofilm formation; Candida albicans; cell-wall biogenesis; yeast colony development
n4:klicoveSlovo
n6:morphogenesis n6:cell-wall%20biogenesis n6:yeast%20colony%20development n6:expression n6:Candida%20albicans n6:biofilm%20formation n6:oscillations n6:death n6:gene n6:congo%20red
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[368537558DBE]
n4:nazevZdroje
PLoS ONE
n4:obor
n22:EB
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
4
n4:projekt
n21:GA13-08605S
n4:rokUplatneniVysledku
n8:2013
n4:svazekPeriodika
8
n4:tvurceVysledku
Kučerová, Helena Váchová, Libuše Palková, Zdena Kuznetsov, Evgeny
n4:wos
000326734500060
n4:zamer
n18:MSM0021620858
s:issn
1932-6203
s:numberOfPages
11
n16:doi
10.1371/journal.pone.0073882
n12:organizacniJednotka
11310