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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10138813%21RIV14-GA0-11310___
rdf:type
skos:Concept n13:Vysledek
rdfs:seeAlso
http://www.biochemj.org/bj/454/bj4540551.htm
dcterms:description
On solid substrates, yeast colonies pass through distinct developmental phases characterized by changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes among others the activation of several plasma membrane transporters and is connected with colony differentiation. In this study, we show that colony cells can use two transport mechanisms to import lactic acid: a %22saturable%22 component of the transport, which requires the presence of functional Jen1p transporter and a %22non-saturable%22 component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. While the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the 2nd acidic phase. This lactate uptake profile correlates with the localisation of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of the non-saturable lactic acid uptake in yeast colonies. On solid substrates, yeast colonies pass through distinct developmental phases characterized by changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes among others the activation of several plasma membrane transporters and is connected with colony differentiation. In this study, we show that colony cells can use two transport mechanisms to import lactic acid: a %22saturable%22 component of the transport, which requires the presence of functional Jen1p transporter and a %22non-saturable%22 component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. While the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the 2nd acidic phase. This lactate uptake profile correlates with the localisation of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of the non-saturable lactic acid uptake in yeast colonies.
dcterms:title
The transport of carboxylic acids and important role of Jen1p transporter during the development of yeast colonies The transport of carboxylic acids and important role of Jen1p transporter during the development of yeast colonies
skos:prefLabel
The transport of carboxylic acids and important role of Jen1p transporter during the development of yeast colonies The transport of carboxylic acids and important role of Jen1p transporter during the development of yeast colonies
skos:notation
RIV/00216208:11310/13:10138813!RIV14-GA0-11310___
n13:predkladatel
n14:orjk%3A11310
n3:aktivita
n4:Z n4:P n4:I
n3:aktivity
I, P(GA204/08/0718), Z(MSM0021620858)
n3:cisloPeriodika
Part: 3, September
n3:dodaniDat
n7:2014
n3:domaciTvurceVysledku
n9:7267347 n9:1892746 n9:1621718
n3:druhVysledku
n8:J
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
111674
n3:idVysledku
RIV/00216208:11310/13:10138813
n3:jazykVysledku
n18:eng
n3:klicovaSlova
yeast colony development; Saccharomyces cerevisiae; monocarboxylic acid import; Jen1p protein; ammonia production
n3:klicoveSlovo
n5:ammonia%20production n5:monocarboxylic%20acid%20import n5:yeast%20colony%20development n5:Saccharomyces%20cerevisiae n5:Jen1p%20protein
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[55E7C032BAA8]
n3:nazevZdroje
Biochemical Journal
n3:obor
n12:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
8
n3:projekt
n17:GA204%2F08%2F0718
n3:rokUplatneniVysledku
n7:2013
n3:svazekPeriodika
454
n3:tvurceVysledku
Paiva, Sandra Casal, Margarida Palková, Zdena Váchová, Libuše Mota, Sandra Strachotová, Dita Kučerová, Helena Hlaváček, Otakar
n3:wos
000329975400019
n3:zamer
n16:MSM0021620858
s:issn
0264-6021
s:numberOfPages
8
n20:doi
10.1042/BJ20120312
n19:organizacniJednotka
11310