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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10133648%21RIV14-MSM-11310___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0065148
dcterms:description
Iron plays a crucial role in metabolism as a key component of catalytic and redox cofactors, such as heme or iron-sulfur clusters in enzymes and electron-transporting or regulatory proteins. Limitation of iron availability by the host is also one of the mechanisms involved in immunity. Pathogens must regulate their protein expression according to the iron concentration in their environment and optimize their metabolic pathways in cases of limitation through the availability of respective cofactors. Trichomonas vaginalis, a sexually transmitted pathogen of humans, requires high iron levels for optimal growth. It is an anaerobe that possesses hydrogenosomes, mitochondrion-related organelles that harbor pathways of energy metabolism and iron-sulfur cluster assembly. We analyzed the proteomes of hydrogenosomes obtained from cells cultivated under iron-rich and iron-deficient conditions employing two-dimensional peptide separation combining IEF and nano-HPLC with quantitative MALDI-MS/MS. We identified 179 proteins, of which 58 were differentially expressed. Iron deficiency led to the upregulation of proteins involved in iron-sulfur cluster assembly and the downregulation of enzymes involved in carbohydrate metabolism. Interestingly, iron affected the expression of only some of multiple protein paralogues, whereas the expression of others was iron independent. This finding indicates a stringent regulation of differentially expressed multiple gene copies in response to changes in the availability of exogenous iron. Iron plays a crucial role in metabolism as a key component of catalytic and redox cofactors, such as heme or iron-sulfur clusters in enzymes and electron-transporting or regulatory proteins. Limitation of iron availability by the host is also one of the mechanisms involved in immunity. Pathogens must regulate their protein expression according to the iron concentration in their environment and optimize their metabolic pathways in cases of limitation through the availability of respective cofactors. Trichomonas vaginalis, a sexually transmitted pathogen of humans, requires high iron levels for optimal growth. It is an anaerobe that possesses hydrogenosomes, mitochondrion-related organelles that harbor pathways of energy metabolism and iron-sulfur cluster assembly. We analyzed the proteomes of hydrogenosomes obtained from cells cultivated under iron-rich and iron-deficient conditions employing two-dimensional peptide separation combining IEF and nano-HPLC with quantitative MALDI-MS/MS. We identified 179 proteins, of which 58 were differentially expressed. Iron deficiency led to the upregulation of proteins involved in iron-sulfur cluster assembly and the downregulation of enzymes involved in carbohydrate metabolism. Interestingly, iron affected the expression of only some of multiple protein paralogues, whereas the expression of others was iron independent. This finding indicates a stringent regulation of differentially expressed multiple gene copies in response to changes in the availability of exogenous iron.
dcterms:title
Iron-induced changes in the proteome of Trichomonas vaginalis hydrogenosomes Iron-induced changes in the proteome of Trichomonas vaginalis hydrogenosomes
skos:prefLabel
Iron-induced changes in the proteome of Trichomonas vaginalis hydrogenosomes Iron-induced changes in the proteome of Trichomonas vaginalis hydrogenosomes
skos:notation
RIV/00216208:11310/13:10133648!RIV14-MSM-11310___
n5:predkladatel
n6:orjk%3A11310
n3:aktivita
n9:I n9:Z
n3:aktivity
I, Z(MSM0021620858)
n3:cisloPeriodika
5
n3:dodaniDat
n19:2014
n3:domaciTvurceVysledku
n4:9972293 n4:9686444 n4:6343171 n4:8660344 n4:1093908 n4:4370880 n4:6517455 n4:9559124
n3:druhVysledku
n12:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
81233
n3:idVysledku
RIV/00216208:11310/13:10133648
n3:jazykVysledku
n21:eng
n3:klicovaSlova
hydrogenosomes; proteomics; iron regulation; Trichomonas vaginalis
n3:klicoveSlovo
n14:proteomics n14:iron%20regulation n14:hydrogenosomes n14:Trichomonas%20vaginalis
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[6CB6ACF58593]
n3:nazevZdroje
PLoS ONE
n3:obor
n7:EE
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
8
n3:rokUplatneniVysledku
n19:2013
n3:svazekPeriodika
8
n3:tvurceVysledku
Rada, Petr Horváthová, Lenka Šedinová, Miroslava Campo Beltran, Neritza Hrdý, Ivan Tachezy, Jan Jedelský, Petr Marcinčiková, Michaela
n3:wos
000319799900188
n3:zamer
n10:MSM0021620858
s:issn
1932-6203
s:numberOfPages
9
n13:doi
10.1371/journal.pone.0065148
n15:organizacniJednotka
11310