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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F13%3A10126893%21RIV14-MSM-11310___
rdf:type
skos:Concept n16:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1039/c2ob26881g
dcterms:description
Modified nucleosides (dA(R)s and dC(R)s) bearing bipyridine or terpyridine ligands attached through an octadiyne linker were prepared by single-step aqueous-phase Sonogashira cross-coupling of 7-iodo-7-deaza-2'-deoxyadenosine and 5-iodo-2'-deoxycytidine with the corresponding bipyridine- or terpyridine-octadiynes and were triphosphorylated to the corresponding nucleoside triphosphates (dA(R)TPs and dC(R)TPs). The modified dN(R)TPs were successfully incorporated into the oligonucleotides by primer extension experiment (PEX) using different DNA polymerases and the PEX products were used for post-synthetic complexation with divalent metal cations. The complexation of these DNAs containing flexibly-tethered ligands was compared with the previously reported ones bearing rigid acetylene-linked ligands suggesting the possible formation of both inter- and intra-strand complexes with Ni2+ or Fe2+. Modified nucleosides (dA(R)s and dC(R)s) bearing bipyridine or terpyridine ligands attached through an octadiyne linker were prepared by single-step aqueous-phase Sonogashira cross-coupling of 7-iodo-7-deaza-2'-deoxyadenosine and 5-iodo-2'-deoxycytidine with the corresponding bipyridine- or terpyridine-octadiynes and were triphosphorylated to the corresponding nucleoside triphosphates (dA(R)TPs and dC(R)TPs). The modified dN(R)TPs were successfully incorporated into the oligonucleotides by primer extension experiment (PEX) using different DNA polymerases and the PEX products were used for post-synthetic complexation with divalent metal cations. The complexation of these DNAs containing flexibly-tethered ligands was compared with the previously reported ones bearing rigid acetylene-linked ligands suggesting the possible formation of both inter- and intra-strand complexes with Ni2+ or Fe2+.
dcterms:title
Synthesis of nucleosides and dNTPs bearing oligopyridine ligands linked through an octadiyne tether, their incorporation into DNA and complexation with transition metal cations Synthesis of nucleosides and dNTPs bearing oligopyridine ligands linked through an octadiyne tether, their incorporation into DNA and complexation with transition metal cations
skos:prefLabel
Synthesis of nucleosides and dNTPs bearing oligopyridine ligands linked through an octadiyne tether, their incorporation into DNA and complexation with transition metal cations Synthesis of nucleosides and dNTPs bearing oligopyridine ligands linked through an octadiyne tether, their incorporation into DNA and complexation with transition metal cations
skos:notation
RIV/00216208:11310/13:10126893!RIV14-MSM-11310___
n16:predkladatel
n17:orjk%3A11310
n3:aktivita
n18:P n18:I
n3:aktivity
I, P(GA203/09/0317)
n3:cisloPeriodika
1
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n11:6659160
n3:druhVysledku
n20:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
109544
n3:idVysledku
RIV/00216208:11310/13:10126893
n3:jazykVysledku
n21:eng
n3:klicovaSlova
terpyridine, metal complexation; functionalized DNA; DNA polymerase; cross-coupling reactions
n3:klicoveSlovo
n4:cross-coupling%20reactions n4:terpyridine n4:metal%20complexation n4:functionalized%20DNA n4:DNA%20polymerase
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[A28D0E036761]
n3:nazevZdroje
Organic and Biomolecular Chemistry
n3:obor
n14:CC
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n15:GA203%2F09%2F0317
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
11
n3:tvurceVysledku
Hocek, Michal Pohl, Radek Kalachová, Lubica Fanfrlík, Jindřich Bednárová, Lucie
n3:wos
000311974300011
s:issn
1477-0520
s:numberOfPages
12
n10:doi
10.1039/c2ob26881g
n12:organizacniJednotka
11310