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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10132873%21RIV13-MSM-11310___
rdf:type
skos:Concept n7:Vysledek
rdfs:seeAlso
http://www.ncbi.nlm.nih.gov/pubmed/22540115
dcterms:description
Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria. Bilirubin is degraded in the human gut by microflora into urobilinoids. In our study we investigated whether the bilirubin-reducing strain of Clostridium perfringens can reduce bilirubin ditaurate (BDT), a bile pigment of some lower vertebrates, without hydrolysis of the taurine moiety. C. perfringes was incubated under anaerobic conditions with BDT; reduction products were quantified by spectrophotometry and separated by TLC. Based on Rf values of BDT reduction products and synthetic urobilinogen ditaurate, three novel taurine-conjugated urobilinoids were identified. It is likely that bilirubin-reducing enzyme(s) serve for the effective disposal of electrons produced by fermentolytic processes in these anaerobic bacteria.
dcterms:title
Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens
skos:prefLabel
Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens
skos:notation
RIV/00216208:11310/12:10132873!RIV13-MSM-11310___
n7:predkladatel
n8:orjk%3A11310
n5:aktivita
n14:P n14:I
n5:aktivity
I, P(GAP206/11/0836)
n5:cisloPeriodika
2
n5:dodaniDat
n16:2013
n5:domaciTvurceVysledku
n10:7066767 n10:9558144
n5:druhVysledku
n13:J
n5:duvernostUdaju
n11:S
n5:entitaPredkladatele
n18:predkladatel
n5:idSjednocenehoVysledku
164489
n5:idVysledku
RIV/00216208:11310/12:10132873
n5:jazykVysledku
n17:eng
n5:klicovaSlova
urobilinoids; intestinal metabolism; bilirubin ditaurate; bile pigments; clostridium perfringens
n5:klicoveSlovo
n6:urobilinoids n6:clostridium%20perfringens n6:bilirubin%20ditaurate n6:bile%20pigments n6:intestinal%20metabolism
n5:kodStatuVydavatele
PL - Polská republika
n5:kontrolniKodProRIV
[980E2093B529]
n5:nazevZdroje
Acta Biochimica Polonica
n5:obor
n9:CE
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
6
n5:projekt
n15:GAP206%2F11%2F0836
n5:rokUplatneniVysledku
n16:2012
n5:svazekPeriodika
59
n5:tvurceVysledku
Koníčková, Renata Vítek, Libor Zelenka, Jaroslav Jirásková, Alena Lešetický, Ladislav Štícha, Martin
n5:wos
000305849100018
s:issn
0001-527X
s:numberOfPages
3
n19:organizacniJednotka
11310