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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10127889%21RIV13-MSM-11310___
rdf:type
skos:Concept n7:Vysledek
rdfs:seeAlso
http://link.springer.com/article/10.1007%2Fs12223-012-0157-y
dcterms:description
The separation of vegetative cells from spores coexisting in Bacillus subtilis stationary phase cultures is a key step when studying both of the subpopulations. Lysozyme treatment was used for breaking down the vegetative cells. Unaffected spores were sedimented and separated by centrifugation. Characterization of the cell-free supernatant showed that it represents membrane and cytoplasmic fractions and, thus, can readily be used for determining their biochemical and microbiological properties. The separation efficacy, selectivity and suitability of the method were verified by observation in microscope, CFU counting, and by analysis of the phospholipid composition as well. The separation of vegetative cells from spores coexisting in Bacillus subtilis stationary phase cultures is a key step when studying both of the subpopulations. Lysozyme treatment was used for breaking down the vegetative cells. Unaffected spores were sedimented and separated by centrifugation. Characterization of the cell-free supernatant showed that it represents membrane and cytoplasmic fractions and, thus, can readily be used for determining their biochemical and microbiological properties. The separation efficacy, selectivity and suitability of the method were verified by observation in microscope, CFU counting, and by analysis of the phospholipid composition as well.
dcterms:title
Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores
skos:prefLabel
Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores Rapid and effective method for the separation of Bacillus subtilis vegetative cells and spores
skos:notation
RIV/00216208:11310/12:10127889!RIV13-MSM-11310___
n7:predkladatel
n8:orjk%3A11310
n4:aktivita
n9:I n9:Z
n4:aktivity
I, Z(MSM0021620858)
n4:cisloPeriodika
5
n4:dodaniDat
n14:2013
n4:domaciTvurceVysledku
n12:4692284 n12:8214018
n4:druhVysledku
n5:J
n4:duvernostUdaju
n13:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
164086
n4:idVysledku
RIV/00216208:11310/12:10127889
n4:jazykVysledku
n20:eng
n4:klicovaSlova
stationary phase; spore separation; phospholipids; lysozyme; Bacillus subtilis
n4:klicoveSlovo
n6:spore%20separation n6:stationary%20phase n6:lysozyme n6:Bacillus%20subtilis n6:phospholipids
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[DBA06ED95062]
n4:nazevZdroje
Folia Microbiologica
n4:obor
n21:EE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:rokUplatneniVysledku
n14:2012
n4:svazekPeriodika
57
n4:tvurceVysledku
Svobodová, Jaroslava Seydlová, Gabriela
n4:wos
000307509300010
n4:zamer
n19:MSM0021620858
s:issn
0015-5632
s:numberOfPages
3
n16:doi
10.1007/s12223-012-0157-y
n17:organizacniJednotka
11310