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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10127523%21RIV13-GA0-11310___
rdf:type
n10:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.actatropica.2011.12.004
dcterms:description
Pooled salivary gland samples are frequently used to ensure the sufficient amount of material for the experiments; however, this could mask an individual variability. Thus, we compared salivary protein profiles in seven colonies of three Phlebotomus species: Phlebotomus sergenti, Phlebotomus perniciosus, and Phlebotomus papatasi. Surprisingly, the individual profiles differed significantly between the colonies as well as between individuals. The highest variability was observed in proteins with molecular masses of 42-46 kDa corresponding to the yellow-related proteins. The phenogram constructed from salivary gland profiles revealed the existence of two main groups in P. sergenti, corresponding well with the geographical origin. The F1 progeny obtained from cross-mating studies between P. sergenti colonies of different geographical origin formed a distinct subgroup within the parental groups. In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed. Pooled salivary gland samples are frequently used to ensure the sufficient amount of material for the experiments; however, this could mask an individual variability. Thus, we compared salivary protein profiles in seven colonies of three Phlebotomus species: Phlebotomus sergenti, Phlebotomus perniciosus, and Phlebotomus papatasi. Surprisingly, the individual profiles differed significantly between the colonies as well as between individuals. The highest variability was observed in proteins with molecular masses of 42-46 kDa corresponding to the yellow-related proteins. The phenogram constructed from salivary gland profiles revealed the existence of two main groups in P. sergenti, corresponding well with the geographical origin. The F1 progeny obtained from cross-mating studies between P. sergenti colonies of different geographical origin formed a distinct subgroup within the parental groups. In P. papatasi, several groups of protein profiles were observed with no relationship to the geographical origin. The biological role of salivary proteins variability is discussed.
dcterms:title
Individual variability of salivary gland proteins in three Phlebotomus species Individual variability of salivary gland proteins in three Phlebotomus species
skos:prefLabel
Individual variability of salivary gland proteins in three Phlebotomus species Individual variability of salivary gland proteins in three Phlebotomus species
skos:notation
RIV/00216208:11310/12:10127523!RIV13-GA0-11310___
n10:predkladatel
n22:orjk%3A11310
n3:aktivita
n15:I n15:S n15:P n15:Z
n3:aktivity
I, P(7E11055), P(GA206/09/0777), P(GA206/09/0822), P(LC06009), S, Z(MSM0021620828)
n3:cisloPeriodika
1
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n19:5208025 n19:1739972 n19:7188056 n19:6447910
n3:druhVysledku
n13:J
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
141220
n3:idVysledku
RIV/00216208:11310/12:10127523
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Anti-Leishmania vaccine; Leishmania transmission; Individual variability; Salivary glands; Phlebotomine sand flies; Phlebotomus
n3:klicoveSlovo
n4:Phlebotomus n4:Phlebotomine%20sand%20flies n4:Salivary%20glands n4:Leishmania%20transmission n4:Individual%20variability n4:Anti-Leishmania%20vaccine
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[BD4E9CC5CC75]
n3:nazevZdroje
Acta Tropica
n3:obor
n18:EG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:projekt
n14:GA206%2F09%2F0777 n14:GA206%2F09%2F0822 n14:7E11055 n14:LC06009
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
122
n3:tvurceVysledku
Rohoušová, Iva Volfová, Věra Nová, Soňa Volf, Petr
n3:wos
000302668600012
n3:zamer
n5:MSM0021620828
s:issn
0001-706X
s:numberOfPages
7
n6:doi
10.1016/j.actatropica.2011.12.004
n17:organizacniJednotka
11310