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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10127426%21RIV13-GA0-11310___
rdf:type
n6:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1603/ME12053
dcterms:description
Biting midges of the genus Forcipomyia (Diptera: Ceratopogonidae) have recently been implicated as vectors of kinetoplastid parasites in the Leishmania enrietti complex. This study assesses susceptibility of one of the few successfully colonized Ceratopogonidae, Culicoides nubeculosus Meigen, to infection with Leishmania parasites infecting humans. While Leishmania infantum initially developed in the midgut of C. nubeculosus until 2 d postfeeding, parasite populations on day 3 were considerably reduced. Despite this, a polymerase chain reaction-based assay continued to indicate presence of L. infantum for up to 7 d after the bloodmeal. These findings are discussed within the wider context of implicating arthropods as vectors of Leishmania and it is suggested that conventional polymerase chain reaction use in vector-competence studies should be accompanied by direct microscopical observations. Biting midges of the genus Forcipomyia (Diptera: Ceratopogonidae) have recently been implicated as vectors of kinetoplastid parasites in the Leishmania enrietti complex. This study assesses susceptibility of one of the few successfully colonized Ceratopogonidae, Culicoides nubeculosus Meigen, to infection with Leishmania parasites infecting humans. While Leishmania infantum initially developed in the midgut of C. nubeculosus until 2 d postfeeding, parasite populations on day 3 were considerably reduced. Despite this, a polymerase chain reaction-based assay continued to indicate presence of L. infantum for up to 7 d after the bloodmeal. These findings are discussed within the wider context of implicating arthropods as vectors of Leishmania and it is suggested that conventional polymerase chain reaction use in vector-competence studies should be accompanied by direct microscopical observations.
dcterms:title
Development of Leishmania Parasites in Culicoides nubeculosus (Diptera: Ceratopogonidae) and Implications for Screening Vector Competence Development of Leishmania Parasites in Culicoides nubeculosus (Diptera: Ceratopogonidae) and Implications for Screening Vector Competence
skos:prefLabel
Development of Leishmania Parasites in Culicoides nubeculosus (Diptera: Ceratopogonidae) and Implications for Screening Vector Competence Development of Leishmania Parasites in Culicoides nubeculosus (Diptera: Ceratopogonidae) and Implications for Screening Vector Competence
skos:notation
RIV/00216208:11310/12:10127426!RIV13-GA0-11310___
n6:predkladatel
n14:orjk%3A11310
n4:aktivita
n5:I n5:S n5:P
n4:aktivity
I, P(7E11055), P(GD206/09/H026), S
n4:cisloPeriodika
5
n4:dodaniDat
n15:2013
n4:domaciTvurceVysledku
n18:6447910 n18:9235949 n18:3628434
n4:druhVysledku
n8:J
n4:duvernostUdaju
n21:S
n4:entitaPredkladatele
n17:predkladatel
n4:idSjednocenehoVysledku
130800
n4:idVysledku
RIV/00216208:11310/12:10127426
n4:jazykVysledku
n11:eng
n4:klicovaSlova
Leishmania transmission; biting midge; vector competence
n4:klicoveSlovo
n9:biting%20midge n9:vector%20competence n9:Leishmania%20transmission
n4:kodStatuVydavatele
US - Spojené státy americké
n4:kontrolniKodProRIV
[7BE93FCD97C8]
n4:nazevZdroje
Journal of Medical Entomology
n4:obor
n7:EG
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n20:7E11055 n20:GD206%2F09%2FH026
n4:rokUplatneniVysledku
n15:2012
n4:svazekPeriodika
49
n4:tvurceVysledku
Volf, Petr Sádlová, Jovana Carpenter, S. Šeblová, Veronika
n4:wos
000308924100001
s:issn
0022-2585
s:numberOfPages
4
n19:doi
10.1603/ME12053
n16:organizacniJednotka
11310