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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10126758%21RIV13-MSM-11310___
rdf:type
n8:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1007/s12010-012-9772-y
dcterms:description
Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of alpha-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. alpha-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown. Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of alpha-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. alpha-Chymotrypsin immobilized to magnetic bead cellulose was used for the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown.
dcterms:title
Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin
skos:prefLabel
Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin
skos:notation
RIV/00216208:11310/12:10126758!RIV13-MSM-11310___
n8:predkladatel
n18:orjk%3A11310
n3:aktivita
n10:I n10:P n10:Z
n3:aktivity
I, P(GA203/09/0857), P(LC06044), Z(AV0Z40500505), Z(MSM0021620806)
n3:cisloPeriodika
2
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n14:6383696
n3:druhVysledku
n7:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n22:predkladatel
n3:idSjednocenehoVysledku
147748
n3:idVysledku
RIV/00216208:11310/12:10126758
n3:jazykVysledku
n4:eng
n3:klicovaSlova
proteomics; microspheres; digestion; trypsin; porcine pepsin A; magnetic bead cellulose; immobilized alpha-chymotrypsin; human gastric juice; enzyme immobilization
n3:klicoveSlovo
n6:microspheres n6:digestion n6:immobilized%20alpha-chymotrypsin n6:enzyme%20immobilization n6:human%20gastric%20juice n6:porcine%20pepsin%20A n6:proteomics n6:trypsin n6:magnetic%20bead%20cellulose
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[A1B1748358E6]
n3:nazevZdroje
Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
n3:obor
n19:CB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n21:GA203%2F09%2F0857 n21:LC06044
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
168
n3:tvurceVysledku
Kučerová, Zdenka Lenfeld, Jiří Přikryl, Petr Horák, Daniel Tichá, Marie
n3:wos
000309224300007
n3:zamer
n11:MSM0021620806 n11:AV0Z40500505
s:issn
0273-2289
s:numberOfPages
11
n16:doi
10.1007/s12010-012-9772-y
n12:organizacniJednotka
11310