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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10126406%21RIV13-GA0-11310___
rdf:type
n3:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1007/s00216-012-6140-2
dcterms:description
A high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) at a boron-doped diamond film electrode with preliminary separation and preconcentration by solid-phase extraction (SPE) has been developed for the determination of 1-hydroxypyrene (1-HP) in human urine. 1-HP is among the most widely used biomarkers of exposure to polycyclic aromatic hydrocarbons. Optimal HPLC-ED conditions have been found: mobile phase methanol-0.05 mol L-1 phosphate buffer pH 5.0 (80:20, v/v), detection potential +1,000 mV versus Ag/AgCl (3 mol L-1 KCl), and flow rate 0.8 mL min(-1). For SPE, LiChrolut(A (R)) RP-18 E cartridges were used. The extraction yield was (87.0 A +/- 5.8) % (n = 5). The concentration dependence of 1-HP was measured in the concentration range from 0.01 to 10 mu mol L-1 (2.18-2,180 mu g L-1) using methanolic solutions resulting from the SPE pretreatment of spiked human urine samples. The limit of detection (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) of the biomarker were 0.013 mu mol L-1 (2.84 mu g L-1) and 0.043 mu mol L-1 (9.39 mu g L-1), respectively, which is sufficient for its determination in the urine of persons exposed to polycyclic aromatic hydrocarbons. A high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) at a boron-doped diamond film electrode with preliminary separation and preconcentration by solid-phase extraction (SPE) has been developed for the determination of 1-hydroxypyrene (1-HP) in human urine. 1-HP is among the most widely used biomarkers of exposure to polycyclic aromatic hydrocarbons. Optimal HPLC-ED conditions have been found: mobile phase methanol-0.05 mol L-1 phosphate buffer pH 5.0 (80:20, v/v), detection potential +1,000 mV versus Ag/AgCl (3 mol L-1 KCl), and flow rate 0.8 mL min(-1). For SPE, LiChrolut(A (R)) RP-18 E cartridges were used. The extraction yield was (87.0 A +/- 5.8) % (n = 5). The concentration dependence of 1-HP was measured in the concentration range from 0.01 to 10 mu mol L-1 (2.18-2,180 mu g L-1) using methanolic solutions resulting from the SPE pretreatment of spiked human urine samples. The limit of detection (signal-to-noise ratio 3) and the limit of quantification (signal-to-noise ratio 10) of the biomarker were 0.013 mu mol L-1 (2.84 mu g L-1) and 0.043 mu mol L-1 (9.39 mu g L-1), respectively, which is sufficient for its determination in the urine of persons exposed to polycyclic aromatic hydrocarbons.
dcterms:title
Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode
skos:prefLabel
Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode Determination of 1-hydroxypyrene in human urine by HPLC with electrochemical detection at a boron-doped diamond film electrode
skos:notation
RIV/00216208:11310/12:10126406!RIV13-GA0-11310___
n3:predkladatel
n4:orjk%3A11310
n6:aktivita
n9:Z n9:I n9:S n9:P
n6:aktivity
I, P(GBP206/12/G151), P(ME10004), S, Z(MSM0021620857)
n6:cisloPeriodika
3
n6:dodaniDat
n12:2013
n6:domaciTvurceVysledku
n11:8805865 n11:1839594 n11:4454472
n6:druhVysledku
n22:J
n6:duvernostUdaju
n8:S
n6:entitaPredkladatele
n19:predkladatel
n6:idSjednocenehoVysledku
130668
n6:idVysledku
RIV/00216208:11310/12:10126406
n6:jazykVysledku
n16:eng
n6:klicovaSlova
Human urine; Solid-phase extraction; Boron-doped diamond film electrode; High-performance liquid chromatography with electrochemical detection; 1-Hydroxypyrene
n6:klicoveSlovo
n7:Boron-doped%20diamond%20film%20electrode n7:Solid-phase%20extraction n7:Human%20urine n7:1-Hydroxypyrene n7:High-performance%20liquid%20chromatography%20with%20electrochemical%20detection
n6:kodStatuVydavatele
DE - Spolková republika Německo
n6:kontrolniKodProRIV
[39F066EC3AA8]
n6:nazevZdroje
Analytical and Bioanalytical Chemistry
n6:obor
n21:CG
n6:pocetDomacichTvurcuVysledku
3
n6:pocetTvurcuVysledku
3
n6:projekt
n13:ME10004 n13:GBP206%2F12%2FG151
n6:rokUplatneniVysledku
n12:2012
n6:svazekPeriodika
404
n6:tvurceVysledku
Vyskočil, Vlastimil Barek, Jiří Yosypchuk, Oksana
n6:wos
000307251900011
n6:zamer
n17:MSM0021620857
s:issn
1618-2642
s:numberOfPages
7
n15:doi
10.1007/s00216-012-6140-2
n20:organizacniJednotka
11310