This HTML5 document contains 60 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n16http://localhost/temp/predkladatel/
n11http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n10http://linked.opendata.cz/resource/domain/vavai/projekt/
n13http://linked.opendata.cz/resource/domain/vavai/subjekt/
n12http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
n5http://linked.opendata.cz/ontology/domain/vavai/riv/
rdfshttp://www.w3.org/2000/01/rdf-schema#
n8http://bibframe.org/vocab/
n21http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216208%3A11310%2F12%3A10126214%21RIV13-GA0-11310___/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n6http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n20http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n14http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10126214%21RIV13-GA0-11310___
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1016/j.toxlet.2012.06.016
dcterms:description
Benzo[a]pyrene (BaP) is a widespread environmental carcinogen activated by cytochrome P450 (P450) enzymes. In Hepatic P450 Reductase Null (HRN) and Reductase Conditional Null (RCN) mice, P450 oxidoreductase (Por) is deleted specifically in hepatocytes, resulting in the loss of essentially all hepatic P450 function. Treatment of HRN mice with a single i.p. or oral dose of BaP (12.5 or 125 mg/kg body weight) resulted in higher DNA adduct levels in liver (up to 10-fold) than in wild-type (WT) mice, indicating that hepatic P450s appear to be more important for BaP detoxification in vivo. Similar results were obtained in RCN mice. We tested whether differences between hepatocytes and non-hepatocytes in P450 activity may underlie the increased liver BaP-DNA binding in HRN mice. Cellular localisation by immunohistochemistry of BaP-DNA adducts showed that HRN mice have ample capacity for formation of BaP-DNA adducts in liver, indicating that the metabolic process does not result in the generation of a reactive species different from that formed in WT mice. However, increased protein expression of cytochrome b(5) in hepatic microsomes of HRN relative to WT mice suggests that cytochrome b5 may modulate the P450-mediated bioactivation of BaP in HRN mice, partially substituting the function of Por. Benzo[a]pyrene (BaP) is a widespread environmental carcinogen activated by cytochrome P450 (P450) enzymes. In Hepatic P450 Reductase Null (HRN) and Reductase Conditional Null (RCN) mice, P450 oxidoreductase (Por) is deleted specifically in hepatocytes, resulting in the loss of essentially all hepatic P450 function. Treatment of HRN mice with a single i.p. or oral dose of BaP (12.5 or 125 mg/kg body weight) resulted in higher DNA adduct levels in liver (up to 10-fold) than in wild-type (WT) mice, indicating that hepatic P450s appear to be more important for BaP detoxification in vivo. Similar results were obtained in RCN mice. We tested whether differences between hepatocytes and non-hepatocytes in P450 activity may underlie the increased liver BaP-DNA binding in HRN mice. Cellular localisation by immunohistochemistry of BaP-DNA adducts showed that HRN mice have ample capacity for formation of BaP-DNA adducts in liver, indicating that the metabolic process does not result in the generation of a reactive species different from that formed in WT mice. However, increased protein expression of cytochrome b(5) in hepatic microsomes of HRN relative to WT mice suggests that cytochrome b5 may modulate the P450-mediated bioactivation of BaP in HRN mice, partially substituting the function of Por.
dcterms:title
Exposure to benzo[a]pyrene of Hepatic Cytochrome P450 Reductase Null (HRN) and P450 Reductase Conditional Null (RCN) mice: Detection of benzo[a]pyrene diol epoxide-DNA adducts by immunohistochemistry and P-32-postlabelling Exposure to benzo[a]pyrene of Hepatic Cytochrome P450 Reductase Null (HRN) and P450 Reductase Conditional Null (RCN) mice: Detection of benzo[a]pyrene diol epoxide-DNA adducts by immunohistochemistry and P-32-postlabelling
skos:prefLabel
Exposure to benzo[a]pyrene of Hepatic Cytochrome P450 Reductase Null (HRN) and P450 Reductase Conditional Null (RCN) mice: Detection of benzo[a]pyrene diol epoxide-DNA adducts by immunohistochemistry and P-32-postlabelling Exposure to benzo[a]pyrene of Hepatic Cytochrome P450 Reductase Null (HRN) and P450 Reductase Conditional Null (RCN) mice: Detection of benzo[a]pyrene diol epoxide-DNA adducts by immunohistochemistry and P-32-postlabelling
skos:notation
RIV/00216208:11310/12:10126214!RIV13-GA0-11310___
n12:predkladatel
n13:orjk%3A11310
n5:aktivita
n17:P n17:I
n5:aktivity
I, P(GAP301/10/0356)
n5:cisloPeriodika
2
n5:dodaniDat
n14:2013
n5:domaciTvurceVysledku
n11:1070177 n11:8486573
n5:druhVysledku
n18:J
n5:duvernostUdaju
n15:S
n5:entitaPredkladatele
n21:predkladatel
n5:idSjednocenehoVysledku
135858
n5:idVysledku
RIV/00216208:11310/12:10126214
n5:jazykVysledku
n20:eng
n5:klicovaSlova
environmental-pollutant; in-vivo; knockout mice; gene-expression; metabolic-activation; anticancer drug ellipticine; polycyclic aromatic hydrocarbon; immunohistochemistry; DNA adducts; Cytochrome P450 oxidoreductase; Cytochrome P450; benzo[a]pyrene
n5:klicoveSlovo
n6:anticancer%20drug%20ellipticine n6:knockout%20mice n6:benzo%5Ba%5Dpyrene n6:immunohistochemistry n6:gene-expression n6:environmental-pollutant n6:Cytochrome%20P450 n6:in-vivo n6:DNA%20adducts n6:metabolic-activation n6:polycyclic%20aromatic%20hydrocarbon n6:Cytochrome%20P450%20oxidoreductase
n5:kodStatuVydavatele
NL - Nizozemsko
n5:kontrolniKodProRIV
[303D969899DB]
n5:nazevZdroje
Toxicology Letters
n5:obor
n19:CE
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
9
n5:projekt
n10:GAP301%2F10%2F0356
n5:rokUplatneniVysledku
n14:2012
n5:svazekPeriodika
213
n5:tvurceVysledku
Stiborová, Marie Sykes, Sarah E. Henderson, Colin J. Wolf, C. Roland Phillips, David H. John, Kaarthik Moserová, Michaela Arlt, Volker M. Poirier, Miriam C.
n5:wos
000308769700004
s:issn
0378-4274
s:numberOfPages
7
n8:doi
10.1016/j.toxlet.2012.06.016
n16:organizacniJednotka
11310