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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10123557%21RIV13-MSM-11310___
rdf:type
n7:Vysledek skos:Concept
rdfs:seeAlso
http://gbe.oxfordjournals.org/content/early/2012/09/12/gbe.evs078.full.pdf+html
dcterms:description
Gene duplication is an important evolutionary mechanism and no eukaryote has more duplicated gene families than the parasitic protist Trichomonas vaginalis. Iron is an essential nutrient for Trichomonas and plays a pivotal role in the establishment of infection, proliferation, and virulence. To gain insight into the role of iron in T. vaginalis gene expression and genome evolution, we screened iron-regulated genes using an oligonucleotide microarray for T. vaginalis and by comparative EST (expressed sequence tag) sequencing of cDNA libraries derived from trichomonads cultivated under iron-rich (+Fe) and iron-restricted (-Fe) conditions. Among 19,000 ESTs from both libraries we identified 336 iron-regulated genes, of which 165 were upregulated under +Fe conditions and 171 under -Fe conditions. The microarray analysis revealed that 195 of 4,950 unique genes were differentially expressed. Of these, 117 genes were upregulated under +Fe conditions, and 78 were upregulated under -Fe conditions. The results of both methods were congruent concerning the regulatory trends and the representation of gene categories. Under +Fe conditions, the expression of proteins involved in carbohydrate metabolism, particularly in the energy metabolism of hydrogenosomes, and in methionine catabolism was increased. The iron-sulphur cluster assembly machinery and certain cysteine proteases are of particular importance among the proteins upregulated under -Fe conditions. A unique feature of the T. vaginalis genome is the retention during evolution of multiple paralogous copies for a majority of all genes. Although the origins and reasons for this gene expansion remain unclear, the retention of multiple gene copies could provide an opportunity to evolve differential expression during growth in variable environmental conditions. For genes whose expression was affected by iron, we found that iron influenced the expression of only some of the paralogous copies, whereas the expression of the other p Gene duplication is an important evolutionary mechanism and no eukaryote has more duplicated gene families than the parasitic protist Trichomonas vaginalis. Iron is an essential nutrient for Trichomonas and plays a pivotal role in the establishment of infection, proliferation, and virulence. To gain insight into the role of iron in T. vaginalis gene expression and genome evolution, we screened iron-regulated genes using an oligonucleotide microarray for T. vaginalis and by comparative EST (expressed sequence tag) sequencing of cDNA libraries derived from trichomonads cultivated under iron-rich (+Fe) and iron-restricted (-Fe) conditions. Among 19,000 ESTs from both libraries we identified 336 iron-regulated genes, of which 165 were upregulated under +Fe conditions and 171 under -Fe conditions. The microarray analysis revealed that 195 of 4,950 unique genes were differentially expressed. Of these, 117 genes were upregulated under +Fe conditions, and 78 were upregulated under -Fe conditions. The results of both methods were congruent concerning the regulatory trends and the representation of gene categories. Under +Fe conditions, the expression of proteins involved in carbohydrate metabolism, particularly in the energy metabolism of hydrogenosomes, and in methionine catabolism was increased. The iron-sulphur cluster assembly machinery and certain cysteine proteases are of particular importance among the proteins upregulated under -Fe conditions. A unique feature of the T. vaginalis genome is the retention during evolution of multiple paralogous copies for a majority of all genes. Although the origins and reasons for this gene expansion remain unclear, the retention of multiple gene copies could provide an opportunity to evolve differential expression during growth in variable environmental conditions. For genes whose expression was affected by iron, we found that iron influenced the expression of only some of the paralogous copies, whereas the expression of the other p
dcterms:title
Transcriptomic identification of iron-regulated and iron-independent gene copies within the heavily duplicated Trichomonas vaginalis genome Transcriptomic identification of iron-regulated and iron-independent gene copies within the heavily duplicated Trichomonas vaginalis genome
skos:prefLabel
Transcriptomic identification of iron-regulated and iron-independent gene copies within the heavily duplicated Trichomonas vaginalis genome Transcriptomic identification of iron-regulated and iron-independent gene copies within the heavily duplicated Trichomonas vaginalis genome
skos:notation
RIV/00216208:11310/12:10123557!RIV13-MSM-11310___
n7:predkladatel
n9:orjk%3A11310
n4:aktivita
n11:Z n11:I n11:P
n4:aktivity
I, P(LC07032), Z(MSM0021620858)
n4:cisloPeriodika
10
n4:dodaniDat
n13:2013
n4:domaciTvurceVysledku
n6:9972293 n6:8660344 n6:4370880 n6:1093908 n6:7444133
n4:druhVysledku
n17:J
n4:duvernostUdaju
n16:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
174857
n4:idVysledku
RIV/00216208:11310/12:10123557
n4:jazykVysledku
n22:eng
n4:klicovaSlova
EST analysis; microarrays; iron; gene duplication
n4:klicoveSlovo
n5:iron n5:EST%20analysis n5:gene%20duplication n5:microarrays
n4:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n4:kontrolniKodProRIV
[397A903EA04F]
n4:nazevZdroje
Genome Biology and Evolution
n4:obor
n15:EE
n4:pocetDomacichTvurcuVysledku
5
n4:pocetTvurcuVysledku
11
n4:projekt
n19:LC07032
n4:rokUplatneniVysledku
n13:2012
n4:svazekPeriodika
4
n4:tvurceVysledku
Tachezy, Jan Shin, Jyh-Wei Basler, Marek Hrdý, Ivan Šafaříková, Lucie Lin, Rose Tang, Petrus Huang, Kuo-Yang Horváthová, Lenka Huang, Po-Jung Campo Beltran, Neritza
n4:wos
000313213800002
n4:zamer
n20:MSM0021620858
s:issn
1759-6653
s:numberOfPages
13
n12:doi
10.1093/gbe/evs078
n21:organizacniJednotka
11310