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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F12%3A10120331%21RIV13-GA0-11310___
rdf:type
n7:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0005273612001538
dcterms:description
Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may forma functional complex. Here,we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasmamembrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p. Each of the three plasma membrane Ato proteins is involved in ammonium signalling and the development of yeast colonies. This suggests that although these proteins are homologous, they do not functionally substitute for each other, but may forma functional complex. Here,we present a detailed combined FRET, FLIM and photobleaching study, which enabled us to detect interactions between Ato proteins found in distinct compartments of yeast cells. We thus show that the proteins Ato1p and Ato2p interact and can form complexes when present in the plasmamembrane. No interaction was detected between Ato1p and Ato3p or Ato2p and Ato3p. In addition, using specially prepared strains, we were able to detect an interaction between molecules of the same Ato protein, namely Ato1p-Ato1p and Ato3p-Ato3p, but not Ato2p-Ato2p.
dcterms:title
Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM) Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)
skos:prefLabel
Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM) Ato protein interactions in yeast plasma membrane revealed by fluorescence lifetime imaging (FLIM)
skos:notation
RIV/00216208:11310/12:10120331!RIV13-GA0-11310___
n7:predkladatel
n22:orjk%3A11310
n3:aktivita
n5:S n5:P n5:I n5:Z
n3:aktivity
I, P(GA204/08/0718), P(LC06063), P(LC531), S, Z(AV0Z40400503), Z(MSM0021620858)
n3:cisloPeriodika
9
n3:dodaniDat
n17:2013
n3:domaciTvurceVysledku
n6:1892746 n6:2558971 n6:1621718 n6:7267347
n3:druhVysledku
n10:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n11:predkladatel
n3:idSjednocenehoVysledku
124018
n3:idVysledku
RIV/00216208:11310/12:10120331
n3:jazykVysledku
n21:eng
n3:klicovaSlova
Donor lifetime; Plasma membrane protein interaction; Homo/hetero di(oligo)mers; FLIM-photobleaching technique; Ammonium exporters Ato1p, Ato2p and Ato3p
n3:klicoveSlovo
n20:Homo%2Fhetero%20di%28oligo%29mers n20:Ato2p%20and%20Ato3p n20:Donor%20lifetime n20:Ammonium%20exporters%20Ato1p n20:FLIM-photobleaching%20technique n20:Plasma%20membrane%20protein%20interaction
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[7DA53F33013F]
n3:nazevZdroje
Biochimica et Biophysica Acta - Biomembranes
n3:obor
n4:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
7
n3:projekt
n12:LC06063 n12:GA204%2F08%2F0718 n12:LC531
n3:rokUplatneniVysledku
n17:2012
n3:svazekPeriodika
1818
n3:tvurceVysledku
Benda, Aleš Holoubek, Aleš Humpolíčková, Jana Kučerová, Helena Váchová, Libuše Strachotová, Dita Palková, Zdena
n3:wos
000306882600006
n3:zamer
n19:AV0Z40400503 n19:MSM0021620858
s:issn
0005-2736
s:numberOfPages
9
n14:doi
10.1016/j.bbamem.2012.05.005
n18:organizacniJednotka
11310