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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F11%3A10108159%21RIV12-MSM-11310___
rdf:type
n12:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1111/j.1948-7134.2011.00106.x
dcterms:description
Sand flies used to have a reputation for being difficult and labour-intensive to breed. Here we summarize our experience with establishment and maintenance of sand fly colonies and their use for infective experiments: techniques for collection and handling wild-caught females, rearing larvae and adults and experimental infections of sand flies by Leishmania using membrane feeding. In addition, we compare major life cycle parameters between various colonies maintained under standard laboratory conditions. The sand fly rearing is tricky but some species can be reared in large numbers with a minimum of space and equipment. Initiation of new colonies from endemic sites is a prerequisite for accurate studies on parasite-vector interaction but it is more difficult step than routine maintenance of colonies already established in laboratory for many generations. Sand flies used to have a reputation for being difficult and labour-intensive to breed. Here we summarize our experience with establishment and maintenance of sand fly colonies and their use for infective experiments: techniques for collection and handling wild-caught females, rearing larvae and adults and experimental infections of sand flies by Leishmania using membrane feeding. In addition, we compare major life cycle parameters between various colonies maintained under standard laboratory conditions. The sand fly rearing is tricky but some species can be reared in large numbers with a minimum of space and equipment. Initiation of new colonies from endemic sites is a prerequisite for accurate studies on parasite-vector interaction but it is more difficult step than routine maintenance of colonies already established in laboratory for many generations.
dcterms:title
Establishment and maintenance of sand fly colonies Establishment and maintenance of sand fly colonies
skos:prefLabel
Establishment and maintenance of sand fly colonies Establishment and maintenance of sand fly colonies
skos:notation
RIV/00216208:11310/11:10108159!RIV12-MSM-11310___
n12:predkladatel
n21:orjk%3A11310
n3:aktivita
n14:Z
n3:aktivity
Z(MSM0021620828)
n3:cisloPeriodika
2.3.2011
n3:dodaniDat
n19:2012
n3:domaciTvurceVysledku
n7:6447910 n7:7188056
n3:druhVysledku
n6:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
197949
n3:idVysledku
RIV/00216208:11310/11:10108159
n3:jazykVysledku
n17:eng
n3:klicovaSlova
infectivity; perniciosus; dogs; transmission; flies; diptera psychodidae; leishmania-infantum; Phlebotomine sandflies
n3:klicoveSlovo
n4:infectivity n4:diptera%20psychodidae n4:flies n4:transmission n4:leishmania-infantum n4:dogs n4:perniciosus n4:Phlebotomine%20sandflies
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[AA7453E7BB02]
n3:nazevZdroje
Journal of Vector Ecology
n3:obor
n16:EG
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:rokUplatneniVysledku
n19:2011
n3:svazekPeriodika
36
n3:tvurceVysledku
Volfová, Věra Volf, Petr
n3:wos
000287927300001
n3:zamer
n11:MSM0021620828
s:issn
1081-1710
s:numberOfPages
9
n18:doi
10.1111/j.1948-7134.2011.00106.x
n20:organizacniJednotka
11310