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Namespace Prefixes

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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F11%3A10107525%21RIV12-GA0-11310___
rdf:type
skos:Concept n9:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1002/jssc.201000869
dcterms:description
Native polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed a charge shift on the proteins resulting into separation of protein species according to their size under physiological conditions. Adsorbed stains could be easily removed after electrophoresis by washing of polyacrylamide gel with buffer and protein complexes could be visualized either by the detection of their enzyme activity or by using a nonspecific protein stain. The specific detection of enzyme activity of glycosidases, lactate dehydrogenase, or phosphatases was shown as an example. Native polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed a charge shift on the proteins resulting into separation of protein species according to their size under physiological conditions. Adsorbed stains could be easily removed after electrophoresis by washing of polyacrylamide gel with buffer and protein complexes could be visualized either by the detection of their enzyme activity or by using a nonspecific protein stain. The specific detection of enzyme activity of glycosidases, lactate dehydrogenase, or phosphatases was shown as an example.
dcterms:title
Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes.
skos:prefLabel
Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes. Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes.
skos:notation
RIV/00216208:11310/11:10107525!RIV12-GA0-11310___
n9:predkladatel
n10:orjk%3A11310
n3:aktivita
n4:P n4:I
n3:aktivity
I, P(GA303/09/1285)
n3:cisloPeriodika
14
n3:dodaniDat
n21:2012
n3:domaciTvurceVysledku
n11:9227121 n11:1455486 n11:6383696 n11:6794033 n11:9976825 n11:6622569
n3:druhVysledku
n5:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
215007
n3:idVysledku
RIV/00216208:11310/11:10107525
n3:jazykVysledku
n6:eng
n3:klicovaSlova
page; form; mannosidase; glucosidase; purification; galactosidase; seeds; Gel-electrophoresis
n3:klicoveSlovo
n7:mannosidase n7:galactosidase n7:purification n7:form n7:seeds n7:glucosidase n7:page n7:Gel-electrophoresis
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[2E3E555325E2]
n3:nazevZdroje
Journal of Separation Science
n3:obor
n19:CE
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n15:GA303%2F09%2F1285
n3:rokUplatneniVysledku
n21:2011
n3:svazekPeriodika
34
n3:tvurceVysledku
Tichá, Marie Kračmerová, Jana Hanzlíková, Eva Tichá, Ivana Dráb, Tomáš Liberda, Jiří
n3:wos
000293588900009
s:issn
1615-9306
s:numberOfPages
4
n18:doi
10.1002/jssc.201000869
n20:organizacniJednotka
11310