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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F11%3A10107221%21RIV12-MSM-11310___
rdf:type
skos:Concept n12:Vysledek
rdfs:seeAlso
http://www.ejes.cz/index.php/ejes/article/view/57
dcterms:description
We describe a method for growing orchids of the genera Dactylorhiza and Ophrys, two European members of the subfamily Orchidoideae, from seeds to mature plants using asymbiotic in vitro cultures and glasshouse pot cultures. Four media were used: two new media 1/4-2 and Mo2 and two modifications of Michl medium (Michl 1988). We also describe a highly efficient technique for seed disinfection using a syringe. We tested the effects of ethanol treatment on Anacmaptis morio (L) R. M. Bateman, Pridgeon & M. W. seeds, sugar media composition on Dactylorhiza majalis (Rchb.) P. F. Hunt & Summerh., Oeceoclades decaryana (H. Perrier ex Guillaumin & Manguin) Garay & Taylor and Ophrys lojaconoi P. Delforge and the effect of kinetin on Dactylorhiza majalis protocorm growth. Sucrose was the best carbon source, while hexose resulted in the inhibition of protocorm development at early stages. The addition of kinetin at 10 mg/l resulted in the formation of the largest protocorms. Ethanol can have positive effect on seed germination when applied for a short time (2 min), while long-time ethanol exposure (60 min) can kill the seeds. We describe a method for growing orchids of the genera Dactylorhiza and Ophrys, two European members of the subfamily Orchidoideae, from seeds to mature plants using asymbiotic in vitro cultures and glasshouse pot cultures. Four media were used: two new media 1/4-2 and Mo2 and two modifications of Michl medium (Michl 1988). We also describe a highly efficient technique for seed disinfection using a syringe. We tested the effects of ethanol treatment on Anacmaptis morio (L) R. M. Bateman, Pridgeon & M. W. seeds, sugar media composition on Dactylorhiza majalis (Rchb.) P. F. Hunt & Summerh., Oeceoclades decaryana (H. Perrier ex Guillaumin & Manguin) Garay & Taylor and Ophrys lojaconoi P. Delforge and the effect of kinetin on Dactylorhiza majalis protocorm growth. Sucrose was the best carbon source, while hexose resulted in the inhibition of protocorm development at early stages. The addition of kinetin at 10 mg/l resulted in the formation of the largest protocorms. Ethanol can have positive effect on seed germination when applied for a short time (2 min), while long-time ethanol exposure (60 min) can kill the seeds.
dcterms:title
European Orchid Cultivation - From Seed to Mature Plant European Orchid Cultivation - From Seed to Mature Plant
skos:prefLabel
European Orchid Cultivation - From Seed to Mature Plant European Orchid Cultivation - From Seed to Mature Plant
skos:notation
RIV/00216208:11310/11:10107221!RIV12-MSM-11310___
n12:predkladatel
n17:orjk%3A11310
n3:aktivita
n4:I
n3:aktivity
I
n3:cisloPeriodika
2
n3:dodaniDat
n14:2012
n3:domaciTvurceVysledku
n5:9562656 n5:4124316 n5:1707094 n5:8713723
n3:druhVysledku
n19:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
198181
n3:idVysledku
RIV/00216208:11310/11:10107221
n3:jazykVysledku
n16:eng
n3:klicovaSlova
kinetin; ethanol; sugars; sowing; In vitro cultivation
n3:klicoveSlovo
n6:In%20vitro%20cultivation n6:sugars n6:sowing n6:kinetin n6:ethanol
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[9B029C4AF1E9]
n3:nazevZdroje
European Journal of Environmental Sciences
n3:obor
n13:ED
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n14:2011
n3:svazekPeriodika
1
n3:tvurceVysledku
Vosolsobě, Stanislav Lipavská, Helena Kmecová, Kateřina Ponert, Jan
s:issn
1805-0174
s:numberOfPages
13
n15:organizacniJednotka
11310