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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F11%3A10105610%21RIV12-GA0-11310___
rdf:type
n9:Vysledek skos:Concept
rdfs:seeAlso
http://botany.natur.cuni.cz/algo/publikace.html
dcterms:description
The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as Jenufa gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as J. perforata and J. minuta, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the Jenufa lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus Golenkinia were suggested both by maximum-likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of J. minuta, indicating the existence of an undescribed Jenufa species occurring in the temperate zone. The diversity of eukaryotic microorganisms is far from fully described, as indicated by the vast number of unassigned genotypes retrieved by environmental sequencing or metagenomics. We isolated several strains of unicellular green algae from algal biofilms growing on tree bark in a Southeast Asian tropical rainforest and determined them to be relatives of an unidentified lineage of environmental 18S rDNA sequences, thus uncovering its cellular identity. Light, confocal, and electron microscope observations and sequencing the 18S rRNA gene revealed that the strains represent two different species within an apparently new genus, described here as Jenufa gen. nov. Both species formed minute coccoid cells with an irregular globular outline, a smooth cell wall, and a single parietal chloroplast without a pyrenoid. The two species, described herein as J. perforata and J. minuta, differed in chloroplast morphology and cell wall structure. Phylogenetic analyses of 18S rRNA gene sequences showed a firm relationship between the two species and placed the Jenufa lineage in an unresolved position within the CS clade (Chlamydomonadales + Sphaeropleales) of the class Chlorophyceae, although possible affinities to the genus Golenkinia were suggested both by maximum-likelihood (ML) and Bayesian methods. Furthermore, two almost identical environmental 18S rDNA sequences from an endolithic microbial community occurring in dolomite rock in the central Alps turned out to be specifically related to, yet apparently distinct from, the sequence of J. minuta, indicating the existence of an undescribed Jenufa species occurring in the temperate zone.
dcterms:title
Jenufa gen. nov.: a new genus of coccoid green algae (Chlorophyceae, incertae sedis) previously recorded by environmental sequencing Jenufa gen. nov.: a new genus of coccoid green algae (Chlorophyceae, incertae sedis) previously recorded by environmental sequencing
skos:prefLabel
Jenufa gen. nov.: a new genus of coccoid green algae (Chlorophyceae, incertae sedis) previously recorded by environmental sequencing Jenufa gen. nov.: a new genus of coccoid green algae (Chlorophyceae, incertae sedis) previously recorded by environmental sequencing
skos:notation
RIV/00216208:11310/11:10105610!RIV12-GA0-11310___
n9:predkladatel
n21:orjk%3A11310
n5:aktivita
n10:V n10:Z n10:P
n5:aktivity
P(GAP506/10/0705), V, Z(MSM0021620828)
n5:cisloPeriodika
4
n5:dodaniDat
n15:2012
n5:domaciTvurceVysledku
n8:2548739 n8:2896729 n8:7077319
n5:druhVysledku
n20:J
n5:duvernostUdaju
n6:S
n5:entitaPredkladatele
n22:predkladatel
n5:idSjednocenehoVysledku
206467
n5:idVysledku
RIV/00216208:11310/11:10105610
n5:jazykVysledku
n18:eng
n5:klicovaSlova
ultrastructure; taxonomy; phylogeny; Jenufa; Chlorophyta; Chlorophyceae; 18S rRNA
n5:klicoveSlovo
n7:Jenufa n7:ultrastructure n7:Chlorophyceae n7:Chlorophyta n7:phylogeny n7:18S%20rRNA n7:taxonomy
n5:kodStatuVydavatele
US - Spojené státy americké
n5:kontrolniKodProRIV
[00BBC718BF49]
n5:nazevZdroje
Journal of Phycology
n5:obor
n16:EF
n5:pocetDomacichTvurcuVysledku
3
n5:pocetTvurcuVysledku
5
n5:projekt
n19:GAP506%2F10%2F0705
n5:rokUplatneniVysledku
n15:2011
n5:svazekPeriodika
47
n5:tvurceVysledku
Eliáš, Marek Němcová, Yvonne Neustupa, Jiří Hodač, Ladislav Škaloud, Pavel
n5:wos
000293096000021
n5:zamer
n17:MSM0021620828
s:issn
0022-3646
s:numberOfPages
11
n13:doi
10.1111/j.1529-8817.2011.01009.x
n12:organizacniJednotka
11310