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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F11%3A10104338%21RIV12-MSM-11310___
rdf:type
skos:Concept n12:Vysledek
rdfs:seeAlso
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3239191/?tool=pubmed
dcterms:description
Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3' splice site (3'ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure prediction and in-line probing showed that this mutation disrupted a stem in the BP-3'ss region. Analyses of various COF1 intron modifications revealed that the secondary structure brought about the reduction of BP to 3'ss distance and masked potential 3'ss. We demonstrated the same structural requisite for the splicing of UBC13 intron. Moreover, RNAfold predicted stable structures for almost all distant BP introns in S. cerevisiae and for selected examples in several other Saccharomycotina species. The employment of intramolecular structure to localize 3'ss for the second splicing step suggests the existence of pre-mRNA structure-based mechanism of 3'ss recognition. Higher order RNA structures can mask splicing signals, loop out exons, or constitute riboswitches all of which contributes to the complexity of splicing regulation. We identified a G to A substitution between branch point (BP) and 3' splice site (3'ss) of Saccharomyces cerevisiae COF1 intron, which dramatically impaired its splicing. RNA structure prediction and in-line probing showed that this mutation disrupted a stem in the BP-3'ss region. Analyses of various COF1 intron modifications revealed that the secondary structure brought about the reduction of BP to 3'ss distance and masked potential 3'ss. We demonstrated the same structural requisite for the splicing of UBC13 intron. Moreover, RNAfold predicted stable structures for almost all distant BP introns in S. cerevisiae and for selected examples in several other Saccharomycotina species. The employment of intramolecular structure to localize 3'ss for the second splicing step suggests the existence of pre-mRNA structure-based mechanism of 3'ss recognition.
dcterms:title
Secondary structure is required for 3' splice site recognition in yeast. Secondary structure is required for 3' splice site recognition in yeast.
skos:prefLabel
Secondary structure is required for 3' splice site recognition in yeast. Secondary structure is required for 3' splice site recognition in yeast.
skos:notation
RIV/00216208:11310/11:10104338!RIV12-MSM-11310___
n12:predkladatel
n17:orjk%3A11310
n3:aktivita
n16:Z n16:S n16:P
n3:aktivity
P(LC07032), S, Z(MSM0021620858)
n3:cisloPeriodika
22
n3:dodaniDat
n6:2012
n3:domaciTvurceVysledku
n13:4231015 n13:2714175 n13:8000964 n13:9107924
n3:druhVysledku
n4:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
228516
n3:idVysledku
RIV/00216208:11310/11:10104338
n3:jazykVysledku
n21:eng
n3:klicovaSlova
distal branch point; splice site recognition; UBC13; COF1; 3' splice site; intron secondary structure
n3:klicoveSlovo
n9:UBC13 n9:splice%20site%20recognition n9:3%27%20splice%20site n9:intron%20secondary%20structure n9:COF1 n9:distal%20branch%20point
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[F94803B30D0D]
n3:nazevZdroje
Nucleic Acids Research
n3:obor
n10:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n20:LC07032
n3:rokUplatneniVysledku
n6:2011
n3:svazekPeriodika
39
n3:tvurceVysledku
Hammann, Christian Půta, František Folk, Petr Valentová, Anna Gahura, Ondřej
n3:wos
000298186000031
n3:zamer
n19:MSM0021620858
s:issn
0305-1048
s:numberOfPages
9
n18:doi
10.1093/nar/gkr662
n7:organizacniJednotka
11310