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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F09%3A10111314%21RIV12-MSM-11310___
rdf:type
n18:Vysledek skos:Concept
dcterms:description
Recombinant plant nucleases R-TBN1 and R-HBN1 were isolated to homogeneity and examined for their antitumor effects and cytotoxicity. Although antiproliferative effects of both recombinant nucleases were not significant on the ML-2 cell culture in vitro, the nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethylene glycol (PEG). Recombinant nucleases were as effective against melanoma tumors as previously studied pine pollen (PN) and mung bean nucleases and their effects were reached at about 10 times lower concentrations compared to the use of bovine seminal RNase (BS-RNase). Because the recombinant nucleases R-HBN1 and R-TBN1 share only 67.4% amino acid identity and showed only partial immunochemical cross-reactivity, their similar anticancerogenic effects can be mainly explained by their catalytical similarity. Both recombinant nucleases showed lower degree of aspermatogenesis compared to BS-RNAse and PN nuclease. Unlike BS-RNase, aspermatogenesis induced by both recombinant nucleases could not be prevented by the homologous antibody complexes. Owing to relatively low cytotoxicity on the one hand, and high efficiency at low protein levels on the other, recombinant plant nucleases R-HBN1 and R-TBN1 appear to be stable biochemical agents that can be targeted as potential antitumor cytostatics. Recombinant plant nucleases R-TBN1 and R-HBN1 were isolated to homogeneity and examined for their antitumor effects and cytotoxicity. Although antiproliferative effects of both recombinant nucleases were not significant on the ML-2 cell culture in vitro, the nucleases were strongly cytostatic in vivo after their administration intravenously as stabilized conjugates with polyethylene glycol (PEG). Recombinant nucleases were as effective against melanoma tumors as previously studied pine pollen (PN) and mung bean nucleases and their effects were reached at about 10 times lower concentrations compared to the use of bovine seminal RNase (BS-RNase). Because the recombinant nucleases R-HBN1 and R-TBN1 share only 67.4% amino acid identity and showed only partial immunochemical cross-reactivity, their similar anticancerogenic effects can be mainly explained by their catalytical similarity. Both recombinant nucleases showed lower degree of aspermatogenesis compared to BS-RNAse and PN nuclease. Unlike BS-RNase, aspermatogenesis induced by both recombinant nucleases could not be prevented by the homologous antibody complexes. Owing to relatively low cytotoxicity on the one hand, and high efficiency at low protein levels on the other, recombinant plant nucleases R-HBN1 and R-TBN1 appear to be stable biochemical agents that can be targeted as potential antitumor cytostatics.
dcterms:title
Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases
skos:prefLabel
Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases Antitumor Effects and Cytotoxicity of Recombinant Plant Nucleases
skos:notation
RIV/00216208:11310/09:10111314!RIV12-MSM-11310___
n3:aktivita
n9:Z n9:P n9:I
n3:aktivity
I, P(GA521/09/1214), Z(AV0Z50450515), Z(AV0Z50510513), Z(MSM0021620808), Z(MSM6046137305), Z(MZ0UHKT2005)
n3:cisloPeriodika
4
n3:dodaniDat
n5:2012
n3:domaciTvurceVysledku
n7:5664772
n3:druhVysledku
n15:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
303537
n3:idVysledku
RIV/00216208:11310/09:10111314
n3:jazykVysledku
n17:eng
n3:klicovaSlova
H. lupulus; L. esculentum; Plant infiltration; Nicotiana benthamina; Spermatogenesis; Tumor xenografts; Human melanoma; Anticarcinogenic and antiproliferative nucleases
n3:klicoveSlovo
n4:H.%20lupulus n4:Human%20melanoma n4:Spermatogenesis n4:L.%20esculentum n4:Anticarcinogenic%20and%20antiproliferative%20nucleases n4:Plant%20infiltration n4:Nicotiana%20benthamina n4:Tumor%20xenografts
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[3F92E6858BF0]
n3:nazevZdroje
Oncology Research
n3:obor
n16:FD
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:projekt
n13:GA521%2F09%2F1214
n3:rokUplatneniVysledku
n5:2009
n3:svazekPeriodika
18
n3:tvurceVysledku
Škvor, Jiří Stehlík, Jan Poučková, Pavla Matoušek, Jaroslav Podzimek, Tomáš Souček, Josef Matoušek, Josef
n3:wos
000272875000004
n3:zamer
n14:AV0Z50510513 n14:AV0Z50450515 n14:MSM6046137305 n14:MZ0UHKT2005 n14:MSM0021620808
s:issn
0965-0407
s:numberOfPages
9
n8:organizacniJednotka
11310