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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F09%3A10001398%21RIV10-GA0-11310___
rdf:type
n18:Vysledek skos:Concept
dcterms:description
Capillary electrophoresis (CE) with contactless conductivity detection (C4D) has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The limit of detection for glycine has been decreased to a value of 0.2 ?M by adding 75% v/v of acetonitrile to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/C4D method makes it possible to determine micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared to a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol. Capillary electrophoresis (CE) with contactless conductivity detection (C4D) has been used to determine the glycine neurotransmitter in periaqueductal gray matter (PAG) of rats. The limit of detection for glycine has been decreased to a value of 0.2 ?M by adding 75% v/v of acetonitrile to the samples and increasing the sample zone introduced to a value of 20% of the overall capillary length. The repeatabilities of the analyte migration times and the zone areas amount to 2.1 and 2.7%, respectively. The optimized CE/C4D method makes it possible to determine micromolar concentrations of glycine in PAG microdialyzates without the necessity of sample derivatization. It follows from a pharmacological study that a local inflammation initiated by an application of carrageenan increased the glycine concentration in the rat PAG seven times, compared to a control. The glycine level in PAG can be decreased and the pain suppressed by administering paracetamol.
dcterms:title
A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection
skos:prefLabel
A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection A determination of submicromolar concentrations of glycine in periaquecuctal gray matter microdialyzates using capillary zone electrophoresis with contactless conductivity detection
skos:notation
RIV/00216208:11310/09:10001398!RIV10-GA0-11310___
n4:aktivita
n9:P n9:Z
n4:aktivity
P(GA203/07/0896), Z(MSM0021620814), Z(MSM0021620816)
n4:cisloPeriodika
19
n4:dodaniDat
n16:2010
n4:domaciTvurceVysledku
n12:5346711
n4:druhVysledku
n11:J
n4:duvernostUdaju
n14:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
301239
n4:idVysledku
RIV/00216208:11310/09:10001398
n4:jazykVysledku
n19:eng
n4:klicovaSlova
Amino acids; Contactless conductivity detection; Neurotransmitters
n4:klicoveSlovo
n15:Contactless%20conductivity%20detection n15:Neurotransmitters n15:Amino%20acids
n4:kodStatuVydavatele
DE - Spolková republika Německo
n4:kontrolniKodProRIV
[58B228D3AADA]
n4:nazevZdroje
Electrophoresis
n4:obor
n13:CB
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
4
n4:projekt
n17:GA203%2F07%2F0896
n4:rokUplatneniVysledku
n16:2009
n4:svazekPeriodika
30
n4:tvurceVysledku
Samcová, Eva Soukupová, Marie Štulík, Karel Tůma, Petr
n4:zamer
n5:MSM0021620816 n5:MSM0021620814
s:issn
0173-0835
s:numberOfPages
6
n6:organizacniJednotka
11310