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Statements

Subject Item
n2:RIV%2F00216208%3A11310%2F09%3A10000724%21RIV10-GA0-11310___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
The highly reduced mitochondria (mitosomes) of Giardia intestinalis are recently discovered organelles for which, it was suggested, iron-sulfur cluster assembly was their only conserved function. However, only an incomplete set of the components required for FeS cluster biogenesis was localized to the mitosomes. Via proteomic analysis of a mitosome-rich cellular fraction together with immunofluorescence microscopy, we identified a novel mitosomal protein homologous to monothiol glutaredoxins containing a CGFS motif at the active site. Sequence analysis revealed the presence of long nonconserved N-terminal extension of 77 amino acids, which was absent in the mature protein. Expression of the complete and N-terminally truncated forms of the glutaredoxin indicated that the extension is involved in glutaredoxin import into mitosomes. However, the mechanism of preprotein processing is unclear, as the mitosomal processing peptidase is unable to cleave this type of extension. The recombinant mature protein The highly reduced mitochondria (mitosomes) of Giardia intestinalis are recently discovered organelles for which, it was suggested, iron-sulfur cluster assembly was their only conserved function. However, only an incomplete set of the components required for FeS cluster biogenesis was localized to the mitosomes. Via proteomic analysis of a mitosome-rich cellular fraction together with immunofluorescence microscopy, we identified a novel mitosomal protein homologous to monothiol glutaredoxins containing a CGFS motif at the active site. Sequence analysis revealed the presence of long nonconserved N-terminal extension of 77 amino acids, which was absent in the mature protein. Expression of the complete and N-terminally truncated forms of the glutaredoxin indicated that the extension is involved in glutaredoxin import into mitosomes. However, the mechanism of preprotein processing is unclear, as the mitosomal processing peptidase is unable to cleave this type of extension. The recombinant mature protein
dcterms:title
The Monothiol Single-Domain Glutaredoxin Is Conserved in the Highly Reduced Mitochondria of Giardia intestinalis The Monothiol Single-Domain Glutaredoxin Is Conserved in the Highly Reduced Mitochondria of Giardia intestinalis
skos:prefLabel
The Monothiol Single-Domain Glutaredoxin Is Conserved in the Highly Reduced Mitochondria of Giardia intestinalis The Monothiol Single-Domain Glutaredoxin Is Conserved in the Highly Reduced Mitochondria of Giardia intestinalis
skos:notation
RIV/00216208:11310/09:10000724!RIV10-GA0-11310___
n3:aktivita
n15:P n15:Z
n3:aktivity
P(GA204/06/0947), P(LC07032), Z(MSM0021620858)
n3:cisloPeriodika
10
n3:dodaniDat
n5:2010
n3:domaciTvurceVysledku
n6:3642763 n6:9972293 n6:8769966 n6:9663223 n6:1093908 n6:9559124 n6:3899586 n6:1915819
n3:druhVysledku
n9:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
327411
n3:idVysledku
RIV/00216208:11310/09:10000724
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Iron-sulfur cluster; proteins; evolution; lamblia; hydrogenosomes; eukaryotes; biogenesis; maturation; mitosomes; frataxin
n3:klicoveSlovo
n4:evolution n4:eukaryotes n4:mitosomes n4:hydrogenosomes n4:Iron-sulfur%20cluster n4:frataxin n4:biogenesis n4:lamblia n4:proteins n4:maturation
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[7A61DB854EC2]
n3:nazevZdroje
Eukaryotic Cell
n3:obor
n12:EE
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
10
n3:projekt
n13:LC07032 n13:GA204%2F06%2F0947
n3:rokUplatneniVysledku
n5:2009
n3:svazekPeriodika
8
n3:tvurceVysledku
Camadro, Jean-Michel Tachezy, Jan Šmíd, Ondřej Doležal, Pavel Hrdý, Ivan Šuťák, Róbert Montagne, Jean-Jacques Rada, Petr Žárský, Vojtěch Pyrih, Jan
n3:wos
000270399300012
n3:zamer
n18:MSM0021620858
s:issn
1535-9778
s:numberOfPages
8
n16:organizacniJednotka
11310