This HTML5 document contains 54 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n9http://localhost/temp/predkladatel/
n17http://linked.opendata.cz/resource/domain/vavai/projekt/
n6http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n20http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
rdfshttp://www.w3.org/2000/01/rdf-schema#
skoshttp://www.w3.org/2004/02/skos/core#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n16http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n19http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216208%3A11160%2F14%3A10146060%21RIV15-MSM-11160___/
n5http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n7http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n13http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n15http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n12http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F14%3A10146060%21RIV15-MSM-11160___
rdf:type
skos:Concept n20:Vysledek
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S073170851300397X
dcterms:description
Two fast, simple, selective and economical sample preparation methods for the determination of entecavir in biological materials available at low amounts are reported. The choice of optimal extraction techniques was performed with regard to analyte hydrophilicity, sample volumes, selectivity, method recovery and rapidity. The compatibility of the eluate with the hydrophilic interaction chromatography (HILIC) mobile phase was crucial to allow the elimination of the evaporation and reconstitution steps and to obtain acceptable peak shapes. Different types of sorbents were employed for the extraction of two biological materials (plasma and plasma ultrafiltrate). The mixed-mode polymeric sorbent MCX was chosen as a suitable one for the solid phase extraction (SPE) of plasma samples. The analytes were eluted with 1 ml of the mixture of 5% ammonium hydroxide in ACN:water (95:5). Protein precipitation (PP) with 1 ml of ACN was used to remove proteins from 500 RI of plasma sample prior to SPE extraction. The microextraction by packed sorbent (MEPS) was employed for the cleaning up of plasma ultrafiltrate samples due to very small volumes available for the analysis. MEPS implemented a novel sorbent based on porous graphitic carbon, semi-automatic analytical syringe and a small volume of sample (50 mu l). The elution step was performed using 100 mu l of the mixture of 5 mM ammonium acetate pH 4.0:ACN (25:75). The MEPS eluate was fully compatible with HILIC mobile phase subsequently used for the analysis of entecavir, unlike SPE eluate, which had to be evaporated and reconstituted in mobile phase. Two fast, simple, selective and economical sample preparation methods for the determination of entecavir in biological materials available at low amounts are reported. The choice of optimal extraction techniques was performed with regard to analyte hydrophilicity, sample volumes, selectivity, method recovery and rapidity. The compatibility of the eluate with the hydrophilic interaction chromatography (HILIC) mobile phase was crucial to allow the elimination of the evaporation and reconstitution steps and to obtain acceptable peak shapes. Different types of sorbents were employed for the extraction of two biological materials (plasma and plasma ultrafiltrate). The mixed-mode polymeric sorbent MCX was chosen as a suitable one for the solid phase extraction (SPE) of plasma samples. The analytes were eluted with 1 ml of the mixture of 5% ammonium hydroxide in ACN:water (95:5). Protein precipitation (PP) with 1 ml of ACN was used to remove proteins from 500 RI of plasma sample prior to SPE extraction. The microextraction by packed sorbent (MEPS) was employed for the cleaning up of plasma ultrafiltrate samples due to very small volumes available for the analysis. MEPS implemented a novel sorbent based on porous graphitic carbon, semi-automatic analytical syringe and a small volume of sample (50 mu l). The elution step was performed using 100 mu l of the mixture of 5 mM ammonium acetate pH 4.0:ACN (25:75). The MEPS eluate was fully compatible with HILIC mobile phase subsequently used for the analysis of entecavir, unlike SPE eluate, which had to be evaporated and reconstituted in mobile phase.
dcterms:title
How to address the sample preparation of hydrophilic compounds: Determination of entecavir in plasma and plasma ultrafiltrate with novel extraction sorbents How to address the sample preparation of hydrophilic compounds: Determination of entecavir in plasma and plasma ultrafiltrate with novel extraction sorbents
skos:prefLabel
How to address the sample preparation of hydrophilic compounds: Determination of entecavir in plasma and plasma ultrafiltrate with novel extraction sorbents How to address the sample preparation of hydrophilic compounds: Determination of entecavir in plasma and plasma ultrafiltrate with novel extraction sorbents
skos:notation
RIV/00216208:11160/14:10146060!RIV15-MSM-11160___
n3:aktivita
n10:S n10:P n10:I
n3:aktivity
I, P(EE2.3.30.0022), S
n3:cisloPeriodika
January
n3:dodaniDat
n12:2015
n3:domaciTvurceVysledku
n6:7555350 n6:8634351 n6:4906268 n6:6252532 n6:4802543 n6:9501045
n3:druhVysledku
n8:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
19798
n3:idVysledku
RIV/00216208:11160/14:10146060
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Hydrophilic compounds; Sample preparation; Porous graphitic carbon; MEPS; Antivirals
n3:klicoveSlovo
n5:Hydrophilic%20compounds n5:Sample%20preparation n5:Porous%20graphitic%20carbon n5:Antivirals n5:MEPS
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[0D0937C2FBF0]
n3:nazevZdroje
Journal of Pharmaceutical and Biomedical Analysis
n3:obor
n15:CB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n17:EE2.3.30.0022
n3:rokUplatneniVysledku
n12:2014
n3:svazekPeriodika
88
n3:tvurceVysledku
Vlčková, Hana Gottvald, Tomáš Janák, Jaroslav Nováková, Lucie Solich, Petr Trejtnar, František
n3:wos
000329532800045
s:issn
0731-7085
s:numberOfPages
8
n16:doi
10.1016/j.jpba.2013.08.034
n9:organizacniJednotka
11160