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Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F14%3A10145990%21RIV15-MSM-11160___
rdf:type
skos:Concept n7:Vysledek
rdfs:seeAlso
http://www.spandidos-publications.com/10.3892/or.2013.2805
dcterms:description
Lung cancer is the leading cause of cancer-related mortality in the world. Chemotherapy has been the mainstay of treatment for advanced non-small cell lung cancer (NSCLC) and platinum-based derivatives have been shown to improve overall survival. The aim of the present study was to investigate the DNA damage [single strand breaks (SSBs) and DNA crosslinks] and DNA repair in peripheral blood lymphocytes in patients with NSCLC treated with platinum derivatives using modified comet assay. Twenty patients in the final (4th) stage of NSCLC and 10 age-corresponding healthy controls participated in the study. Alkaline comet assay was performed according to the appropriate protocol. The DNA base excision repair (BER) activity of the controls was significantly higher compared to that of cancer patients, and the activity of DNA nucleotide excision repair (NER) was almost at the same level both in controls and patients. We observed changes in the amount of SSBs and DNA crosslinks during the course of chemotherapy. We found a significantly higher level of SSBs immediately after administration of chemotherapy. Similarly, we found the highest incidence of DNA crosslinks immediately or 1 day after chemotherapy (compared to measurement before chemotherapy). Moreover, we compared the levels of DNA repair in patients who survived chemotherapy with those in patients who died in the course of chemotherapy: the activity of BER was higher in the case of surviving patients, while the levels of NER were essentially the same. The data arising from the present study confirm the findings of other studies dealing with DNA damage and repair in cancer patients treated with chemotherapy. Moreover, our results indicated that despite the fact that cisplatin-DNA adducts are removed by the NER pathway, BER may also play a role in the clinical status of patients and their survival. Lung cancer is the leading cause of cancer-related mortality in the world. Chemotherapy has been the mainstay of treatment for advanced non-small cell lung cancer (NSCLC) and platinum-based derivatives have been shown to improve overall survival. The aim of the present study was to investigate the DNA damage [single strand breaks (SSBs) and DNA crosslinks] and DNA repair in peripheral blood lymphocytes in patients with NSCLC treated with platinum derivatives using modified comet assay. Twenty patients in the final (4th) stage of NSCLC and 10 age-corresponding healthy controls participated in the study. Alkaline comet assay was performed according to the appropriate protocol. The DNA base excision repair (BER) activity of the controls was significantly higher compared to that of cancer patients, and the activity of DNA nucleotide excision repair (NER) was almost at the same level both in controls and patients. We observed changes in the amount of SSBs and DNA crosslinks during the course of chemotherapy. We found a significantly higher level of SSBs immediately after administration of chemotherapy. Similarly, we found the highest incidence of DNA crosslinks immediately or 1 day after chemotherapy (compared to measurement before chemotherapy). Moreover, we compared the levels of DNA repair in patients who survived chemotherapy with those in patients who died in the course of chemotherapy: the activity of BER was higher in the case of surviving patients, while the levels of NER were essentially the same. The data arising from the present study confirm the findings of other studies dealing with DNA damage and repair in cancer patients treated with chemotherapy. Moreover, our results indicated that despite the fact that cisplatin-DNA adducts are removed by the NER pathway, BER may also play a role in the clinical status of patients and their survival.
dcterms:title
DNA crosslinks, DNA damage and repair in peripheral blood lymphocytes of non-small cell lung cancer patients treated with platinum derivatives DNA crosslinks, DNA damage and repair in peripheral blood lymphocytes of non-small cell lung cancer patients treated with platinum derivatives
skos:prefLabel
DNA crosslinks, DNA damage and repair in peripheral blood lymphocytes of non-small cell lung cancer patients treated with platinum derivatives DNA crosslinks, DNA damage and repair in peripheral blood lymphocytes of non-small cell lung cancer patients treated with platinum derivatives
skos:notation
RIV/00216208:11160/14:10145990!RIV15-MSM-11160___
n5:aktivita
n11:V n11:S n11:I
n5:aktivity
I, S, V
n5:cisloPeriodika
1
n5:dodaniDat
n14:2015
n5:domaciTvurceVysledku
n16:5618444 n16:3868648
n5:druhVysledku
n15:J
n5:duvernostUdaju
n9:S
n5:entitaPredkladatele
n8:predkladatel
n5:idSjednocenehoVysledku
11994
n5:idVysledku
RIV/00216208:11160/14:10145990
n5:jazykVysledku
n17:eng
n5:klicovaSlova
non-small cell lung cancer; DNA repair; DNA damage; comet assay
n5:klicoveSlovo
n10:DNA%20repair n10:non-small%20cell%20lung%20cancer n10:comet%20assay n10:DNA%20damage
n5:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n5:kontrolniKodProRIV
[EBC28EBF5ADF]
n5:nazevZdroje
Oncology Reports
n5:obor
n19:FD
n5:pocetDomacichTvurcuVysledku
2
n5:pocetTvurcuVysledku
6
n5:rokUplatneniVysledku
n14:2014
n5:svazekPeriodika
31
n5:tvurceVysledku
Hrnčiarik, Michal Zadák, Zdeněk Hronek, Miloslav Hrnčiariková, Dana Fikrová, Petra Štětina, Rudolf
n5:wos
000330788100053
s:issn
1021-335X
s:numberOfPages
6
n4:doi
10.3892/or.2013.2805
n18:organizacniJednotka
11160