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Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F13%3A10194157%21RIV14-GA0-11160___
rdf:type
n9:Vysledek skos:Concept
rdfs:seeAlso
http://pubs.acs.org/doi/full/10.1021/la4037474
dcterms:description
Ceramides are essential constituents of the skin barrier that allow humans to live on dry land. Reduced levels of ceramides have been associated with skin diseases, e.g., atopic dermatitis. However, the structural requirements and mechanisms of action of ceramides are not fully understood. Here, we report the effects of ceramide acyl chain length on the permeabilities and biophysics of lipid membranes composed of ceramides (or free sphingosine), fatty acids, cholesterol, and cholesterol sulfate. Short-chain ceramides increased the permeability of the lipid membranes compared to a long-chain ceramide with maxima at 4-6 carbons in the acyl. By a combination of differential scanning calorimetry, Fourier transform infrared spectroscopy, X-ray diffraction, Langmuir monolayers, and atomic force microscopy, we found that the reason for this effect in short ceramides was a lower proportion of tight orthorhombic packing and phase separation of continuous short ceramide-enriched domains with shorter lamellar periodicity compared to native long ceramides. Thus, long acyl chains in ceramides are essential for the formation of tightly packed impermeable lipid lamellae. Moreover, the model skin lipid membranes are a valuable tool to study the relationships between the lipid structure and composition, lipid organization, and the membrane permeability. Ceramides are essential constituents of the skin barrier that allow humans to live on dry land. Reduced levels of ceramides have been associated with skin diseases, e.g., atopic dermatitis. However, the structural requirements and mechanisms of action of ceramides are not fully understood. Here, we report the effects of ceramide acyl chain length on the permeabilities and biophysics of lipid membranes composed of ceramides (or free sphingosine), fatty acids, cholesterol, and cholesterol sulfate. Short-chain ceramides increased the permeability of the lipid membranes compared to a long-chain ceramide with maxima at 4-6 carbons in the acyl. By a combination of differential scanning calorimetry, Fourier transform infrared spectroscopy, X-ray diffraction, Langmuir monolayers, and atomic force microscopy, we found that the reason for this effect in short ceramides was a lower proportion of tight orthorhombic packing and phase separation of continuous short ceramide-enriched domains with shorter lamellar periodicity compared to native long ceramides. Thus, long acyl chains in ceramides are essential for the formation of tightly packed impermeable lipid lamellae. Moreover, the model skin lipid membranes are a valuable tool to study the relationships between the lipid structure and composition, lipid organization, and the membrane permeability.
dcterms:title
Ceramides in the Skin Lipid Membranes: Length Matters Ceramides in the Skin Lipid Membranes: Length Matters
skos:prefLabel
Ceramides in the Skin Lipid Membranes: Length Matters Ceramides in the Skin Lipid Membranes: Length Matters
skos:notation
RIV/00216208:11160/13:10194157!RIV14-GA0-11160___
n9:predkladatel
n14:orjk%3A11160
n3:aktivita
n12:I n12:S n12:P
n3:aktivity
I, P(GA13-23891S), P(GAP207/11/0365), S
n3:cisloPeriodika
50
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n5:5705347 n5:5911516 n5:4396847 n5:3938107 n5:6128688 n5:3216691 n5:4188365 n5:7867328
n3:druhVysledku
n17:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
64599
n3:idVysledku
RIV/00216208:11160/13:10194157
n3:jazykVysledku
n15:eng
n3:klicovaSlova
langmuir monolayers; molecular-organization; in-vitro; permeability barrier; long-chain; barrier function; vitro percutaneous penetration; X-ray-diffraction; stratum-corneum lipids; short-chain ceramides
n3:klicoveSlovo
n6:barrier%20function n6:in-vitro n6:langmuir%20monolayers n6:molecular-organization n6:permeability%20barrier n6:stratum-corneum%20lipids n6:short-chain%20ceramides n6:long-chain n6:X-ray-diffraction n6:vitro%20percutaneous%20penetration
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[E60B17770683]
n3:nazevZdroje
Langmuir : the ACS journal of surfaces and colloids
n3:obor
n4:FR
n3:pocetDomacichTvurcuVysledku
8
n3:pocetTvurcuVysledku
11
n3:projekt
n8:GAP207%2F11%2F0365 n8:GA13-23891S
n3:rokUplatneniVysledku
n13:2013
n3:svazekPeriodika
29
n3:tvurceVysledku
Gooris, Gert Janůšová, Barbora Školová, Barbora Berka, Pavel Hrabálek, Alexandr Zbytovská, Jarmila Palát, Karel Vávrová, Kateřina Roh, Jaroslav Slepička, Petr Bouwstra, Joke
n3:wos
000328797500019
s:issn
0743-7463
s:numberOfPages
10
n20:doi
10.1021/la4037474
n21:organizacniJednotka
11160