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Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F13%3A10146057%21RIV14-MSM-11160___
rdf:type
n8:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0021967312013386
dcterms:description
The development of bioanalytical methods has become more and more challenging over the past years due to very demanding requirements in terms of method reliability, sensitivity, speed of analysis and sample throughput. LC-MS/MS has established itself as a method of choice for routine analysis of biological materials. A development of such method consists of several steps including sample preparation and clean-up step, efficient chromatographic separation, sensitive and selective detection of analytes in complex matrices, a choice of convenient data processing and calibration approach and finally method validation. Each of these steps has its own constraints and challenges, which are discussed in detail in this review. Novel and modern approaches in sample preparation, chromatography and detection are especially emphasized. Attention is paid to proper calibration approach and matrix effects that can seriously affect method accuracy and precision. The development of bioanalytical methods has become more and more challenging over the past years due to very demanding requirements in terms of method reliability, sensitivity, speed of analysis and sample throughput. LC-MS/MS has established itself as a method of choice for routine analysis of biological materials. A development of such method consists of several steps including sample preparation and clean-up step, efficient chromatographic separation, sensitive and selective detection of analytes in complex matrices, a choice of convenient data processing and calibration approach and finally method validation. Each of these steps has its own constraints and challenges, which are discussed in detail in this review. Novel and modern approaches in sample preparation, chromatography and detection are especially emphasized. Attention is paid to proper calibration approach and matrix effects that can seriously affect method accuracy and precision.
dcterms:title
Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis
skos:prefLabel
Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis
skos:notation
RIV/00216208:11160/13:10146057!RIV14-MSM-11160___
n8:predkladatel
n13:orjk%3A11160
n3:aktivita
n12:I
n3:aktivity
I
n3:cisloPeriodika
May
n3:dodaniDat
n4:2014
n3:domaciTvurceVysledku
n20:4906268
n3:druhVysledku
n9:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
64880
n3:idVysledku
RIV/00216208:11160/13:10146057
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Method validation; Matrix effects; LC-MS; UHPLC; Sample preparation; Bioanalysis
n3:klicoveSlovo
n6:Sample%20preparation n6:Matrix%20effects n6:LC-MS n6:Bioanalysis n6:UHPLC n6:Method%20validation
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[C129E2ABD239]
n3:nazevZdroje
Journal of Chromatography A
n3:obor
n16:CB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
1
n3:rokUplatneniVysledku
n4:2013
n3:svazekPeriodika
1292
n3:tvurceVysledku
Nováková, Lucie
n3:wos
000319237100004
s:issn
0021-9673
s:numberOfPages
13
n17:doi
10.1016/j.chroma.2012.08.087
n19:organizacniJednotka
11160