This HTML5 document contains 53 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n13http://localhost/temp/predkladatel/
n17http://linked.opendata.cz/resource/domain/vavai/projekt/
n5http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n19http://linked.opendata.cz/resource/domain/vavai/subjekt/
n14http://linked.opendata.cz/ontology/domain/vavai/
n21http://linked.opendata.cz/resource/domain/vavai/zamer/
shttp://schema.org/
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
rdfshttp://www.w3.org/2000/01/rdf-schema#
skoshttp://www.w3.org/2004/02/skos/core#
n15http://bibframe.org/vocab/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
n7http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216208%3A11160%2F13%3A10145771%21RIV14-MSM-11160___/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n10http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n16http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n12http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n20http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F13%3A10145771%21RIV14-MSM-11160___
rdf:type
skos:Concept n14:Vysledek
rdfs:seeAlso
http://jab.zsf.jcu.cz/11_1/pavlicek.pdf
dcterms:description
Equipment for fast and accurate detection of organophosphate nerve agents is developed and tested. The method is based on the spectrophotometric monitoring of the enzyme activity of butyrylcholinesterase after its contact with air in a special absorption unit (a %22scrubber%22) developed for the purpose. The scrubber was made from a glass tube filled with glass beads (diam. 3 mm) and filled with approx. 5 ml of butyrylcholinesterase in a phosphate buffer of pH 7.4. The air sample was bubbled through this solution for 20 s at a flow rate of 80 l hour(-1). Thereafter 8 mu l of the enzyme solution were aspirated into the micro-SIA-LOV analyzer and the activity of the enzymes were evaluated by using Ellman's reagent, i.e. 2.5 mmol l(-1) butyrylthiocholine iodide and 0.25 mmol 5,5'-dithiobis (2-nitrobenzoic acid). The absorbance of the coloured reaction product was measured at 412 nm after the reaction time of 60 s. The residue of the absorption liquid was washed away from the absorber and the system was washed with the enzyme solution prior to next analysis. The contaminated air caused partial inhibition of the enzyme activity of the absorption liquid. The activity of the contaminated sample was compared with the activity of the unaffected enzyme (blank measurement). The analysis was controlled by two PCs. The effect of the concentration of analyte in the absorption liquid on the enzyme activity was tested for 10(-5)-10(-9) mol l(-1) sarin. A single analysis (including the absorption step) took <130 s. Equipment for fast and accurate detection of organophosphate nerve agents is developed and tested. The method is based on the spectrophotometric monitoring of the enzyme activity of butyrylcholinesterase after its contact with air in a special absorption unit (a %22scrubber%22) developed for the purpose. The scrubber was made from a glass tube filled with glass beads (diam. 3 mm) and filled with approx. 5 ml of butyrylcholinesterase in a phosphate buffer of pH 7.4. The air sample was bubbled through this solution for 20 s at a flow rate of 80 l hour(-1). Thereafter 8 mu l of the enzyme solution were aspirated into the micro-SIA-LOV analyzer and the activity of the enzymes were evaluated by using Ellman's reagent, i.e. 2.5 mmol l(-1) butyrylthiocholine iodide and 0.25 mmol 5,5'-dithiobis (2-nitrobenzoic acid). The absorbance of the coloured reaction product was measured at 412 nm after the reaction time of 60 s. The residue of the absorption liquid was washed away from the absorber and the system was washed with the enzyme solution prior to next analysis. The contaminated air caused partial inhibition of the enzyme activity of the absorption liquid. The activity of the contaminated sample was compared with the activity of the unaffected enzyme (blank measurement). The analysis was controlled by two PCs. The effect of the concentration of analyte in the absorption liquid on the enzyme activity was tested for 10(-5)-10(-9) mol l(-1) sarin. A single analysis (including the absorption step) took <130 s.
dcterms:title
Automated detection of organophosphate warfare gases (nerve agents) in air based on micro-SIA - lab-on-valve system Automated detection of organophosphate warfare gases (nerve agents) in air based on micro-SIA - lab-on-valve system
skos:prefLabel
Automated detection of organophosphate warfare gases (nerve agents) in air based on micro-SIA - lab-on-valve system Automated detection of organophosphate warfare gases (nerve agents) in air based on micro-SIA - lab-on-valve system
skos:notation
RIV/00216208:11160/13:10145771!RIV14-MSM-11160___
n14:predkladatel
n19:orjk%3A11160
n4:aktivita
n6:Z n6:I n6:S n6:P
n4:aktivity
I, P(OVUOFVZ200801), S, Z(MSM0021620822)
n4:cisloPeriodika
1
n4:dodaniDat
n20:2014
n4:domaciTvurceVysledku
n5:6095607 n5:9992340 n5:5813581
n4:druhVysledku
n12:J
n4:duvernostUdaju
n9:S
n4:entitaPredkladatele
n7:predkladatel
n4:idSjednocenehoVysledku
62564
n4:idVysledku
RIV/00216208:11160/13:10145771
n4:jazykVysledku
n16:eng
n4:klicovaSlova
sarin; nerve agent; cholinesterase; organophosphate; lab-on-valve; sequential injection analysis
n4:klicoveSlovo
n10:nerve%20agent n10:cholinesterase n10:organophosphate n10:sarin n10:lab-on-valve n10:sequential%20injection%20analysis
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[955FDD8060EF]
n4:nazevZdroje
Journal of Applied Biomedicine
n4:obor
n18:CB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
4
n4:projekt
n17:OVUOFVZ200801
n4:rokUplatneniVysledku
n20:2013
n4:svazekPeriodika
11
n4:tvurceVysledku
Foltýn, Martin Cabal, Jiří Pavlíček, Ondřej Polášek, Miroslav
n4:wos
000312363000004
n4:zamer
n21:MSM0021620822
s:issn
1214-021X
s:numberOfPages
6
n15:doi
10.2478/v10136-012-0016-9
n13:organizacniJednotka
11160