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Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F13%3A10145719%21RIV14-MSM-11160___
rdf:type
skos:Concept n16:Vysledek
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0039914012008673
dcterms:description
This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of this work was to extend the chromatographic capabilities of the SIC system. Despite the particle-packed columns reaching system pressures of {= 610 PSI, their conditions matched those of a commercially produced and optimised SIC system. The selectivity of each of the tested columns, Ascentis (R) Express RP-Amide, Ascentis (R) Express Phenyl-Hexyl and Ascentis (R) Express C18 (30 mm x 4.6 mm, core-shell particle size 2.7 mu m), was compared by their ability to separate seven phenolic acids that are secondary metabolite substances widely distributed in plants. The separations of all of the components were performed by isocratic elution using binary mobile phases composed of acetonitrile and 0.065% phosphoric acid at pH 2.4 (a specific ratio was used for each column) at a flow-rate of 0.60 mL/min. The volume of the mobile phase was 3.8 mL for each separation. The injection volume of the sample was 10 mu L for each separation. The UV detection wavelengths were set to 250, 280 and 325 nm. The RP-Amide column provided the highest chromatographic resolution and allowed for complete baseline separation of protocatechuic, syringic, vanillic, ferulic, sinapinic, p-coumaric and o-coumaric acids. The analytical parameters were a LOD of 0.3 mg L-1, a LOQof 1.0 mg L-1, a calibration range of 1.0-50.0 (100.0) mg L-1 (r > 0.997) and a system precision of 10 mg L-1 with a RSD {= 1.65%. The high performance of the chromatography process with the RP-Amide column under optimised conditions was highlighted and well documented (HETP values {= 10 mu m, peak symmetry {= 1.33, resolution }= 1.87 and time for one analysis <8.0 min). The results of these experiments confirmed the benefits of extending chromatographic selectivity using core-shell particle column technology in a SIC manifold. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of this work was to extend the chromatographic capabilities of the SIC system. Despite the particle-packed columns reaching system pressures of {= 610 PSI, their conditions matched those of a commercially produced and optimised SIC system. The selectivity of each of the tested columns, Ascentis (R) Express RP-Amide, Ascentis (R) Express Phenyl-Hexyl and Ascentis (R) Express C18 (30 mm x 4.6 mm, core-shell particle size 2.7 mu m), was compared by their ability to separate seven phenolic acids that are secondary metabolite substances widely distributed in plants. The separations of all of the components were performed by isocratic elution using binary mobile phases composed of acetonitrile and 0.065% phosphoric acid at pH 2.4 (a specific ratio was used for each column) at a flow-rate of 0.60 mL/min. The volume of the mobile phase was 3.8 mL for each separation. The injection volume of the sample was 10 mu L for each separation. The UV detection wavelengths were set to 250, 280 and 325 nm. The RP-Amide column provided the highest chromatographic resolution and allowed for complete baseline separation of protocatechuic, syringic, vanillic, ferulic, sinapinic, p-coumaric and o-coumaric acids. The analytical parameters were a LOD of 0.3 mg L-1, a LOQof 1.0 mg L-1, a calibration range of 1.0-50.0 (100.0) mg L-1 (r > 0.997) and a system precision of 10 mg L-1 with a RSD {= 1.65%. The high performance of the chromatography process with the RP-Amide column under optimised conditions was highlighted and well documented (HETP values {= 10 mu m, peak symmetry {= 1.33, resolution }= 1.87 and time for one analysis <8.0 min). The results of these experiments confirmed the benefits of extending chromatographic selectivity using core-shell particle column technology in a SIC manifold.
dcterms:title
Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids
skos:prefLabel
Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids Advantages of core-shell particle columns in Sequential Injection Chromatography for determination of phenolic acids
skos:notation
RIV/00216208:11160/13:10145719!RIV14-MSM-11160___
n16:predkladatel
n17:orjk%3A11160
n3:aktivita
n8:S n8:I
n3:aktivity
I, S
n3:cisloPeriodika
January
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n4:9769501 n4:2796899 n4:7555350 n4:7664370 n4:9088776
n3:druhVysledku
n9:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
59661
n3:idVysledku
RIV/00216208:11160/13:10145719
n3:jazykVysledku
n20:eng
n3:klicovaSlova
Phenolic acids; Columns comparison; Phenyl-Hexyl; RP-C18; RP-Amide; Core-shell particle column; Sequential Injection Chromatography
n3:klicoveSlovo
n5:Sequential%20Injection%20Chromatography n5:Phenolic%20acids n5:RP-Amide n5:Columns%20comparison n5:RP-C18 n5:Phenyl-Hexyl n5:Core-shell%20particle%20column
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[B86AF7A14114]
n3:nazevZdroje
Talanta
n3:obor
n6:FR
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n13:2013
n3:svazekPeriodika
103
n3:tvurceVysledku
Solich, Petr Vacková, Jana Šrámková, Ivana Chocholouš, Petr Šatínský, Dalibor
n3:wos
000314010600032
s:issn
0039-9140
s:numberOfPages
7
n10:doi
10.1016/j.talanta.2012.10.036
n18:organizacniJednotka
11160