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Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F13%3A10144150%21RIV14-MSM-11160___
rdf:type
skos:Concept n12:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.4149/neo_2013_053
dcterms:description
Platinum-based chemotherapeutic agents induce the formation of crosslinks in DNA, which are accepted as being responsible for the cytotoxicity of these agents. In this study, we used a modification of the alkaline comet assay for detection of the presence of DNA crosslinks in vitro caused by cisplatin, and in peripheral lymphocytes of patients with non-small cell lung carcinoma undergoing chemotherapy with platinum derivatives. The comet technique modified for the detection of DNA crosslinks was calibrated in vitro by treating HeLa cells and human lymphocytes from healthy donors with different concentrations of cisplatin. A cisplatin dose-dependent formation of DNA crosslinks was observed in in vitro measurements using 10-200 mu M concentrations of cisplatin. Lymphocytes from cancer patients were also assayed for the formation and repair of DNA crosslinks. Evidence of crosslink formation and repair was observed in peripheral blood lymphocytes of all cancer patients in this study, although some inter-individual differences were observed in the response to chemotherapy and in repair of DNA crosslinks. We propose that monitoring the number of DNA crosslinks in peripheral blood lymphocytes might be a quick and sensitive method for monitoring a patient's sensitivity to this agent. Modification of the method by incubation of analysed cells with styrene oxide before crosslink analysis by comet assay extends the use of the method also to laboratories which have no facilities to use ionizing irradiation for introducing DNA breaks into the cells. Platinum-based chemotherapeutic agents induce the formation of crosslinks in DNA, which are accepted as being responsible for the cytotoxicity of these agents. In this study, we used a modification of the alkaline comet assay for detection of the presence of DNA crosslinks in vitro caused by cisplatin, and in peripheral lymphocytes of patients with non-small cell lung carcinoma undergoing chemotherapy with platinum derivatives. The comet technique modified for the detection of DNA crosslinks was calibrated in vitro by treating HeLa cells and human lymphocytes from healthy donors with different concentrations of cisplatin. A cisplatin dose-dependent formation of DNA crosslinks was observed in in vitro measurements using 10-200 mu M concentrations of cisplatin. Lymphocytes from cancer patients were also assayed for the formation and repair of DNA crosslinks. Evidence of crosslink formation and repair was observed in peripheral blood lymphocytes of all cancer patients in this study, although some inter-individual differences were observed in the response to chemotherapy and in repair of DNA crosslinks. We propose that monitoring the number of DNA crosslinks in peripheral blood lymphocytes might be a quick and sensitive method for monitoring a patient's sensitivity to this agent. Modification of the method by incubation of analysed cells with styrene oxide before crosslink analysis by comet assay extends the use of the method also to laboratories which have no facilities to use ionizing irradiation for introducing DNA breaks into the cells.
dcterms:title
Detection of DNA crosslinks in peripheral lymphocytes isolated from patients treated with platinum derivates using modified comet assay Detection of DNA crosslinks in peripheral lymphocytes isolated from patients treated with platinum derivates using modified comet assay
skos:prefLabel
Detection of DNA crosslinks in peripheral lymphocytes isolated from patients treated with platinum derivates using modified comet assay Detection of DNA crosslinks in peripheral lymphocytes isolated from patients treated with platinum derivates using modified comet assay
skos:notation
RIV/00216208:11160/13:10144150!RIV14-MSM-11160___
n12:predkladatel
n13:orjk%3A11160
n4:aktivita
n6:S n6:I
n4:aktivity
I, S
n4:cisloPeriodika
4
n4:dodaniDat
n14:2014
n4:domaciTvurceVysledku
n9:5618444 n9:3868648
n4:druhVysledku
n5:J
n4:duvernostUdaju
n20:S
n4:entitaPredkladatele
n15:predkladatel
n4:idSjednocenehoVysledku
68726
n4:idVysledku
RIV/00216208:11160/13:10144150
n4:jazykVysledku
n16:eng
n4:klicovaSlova
cisplatin; DNA crosslinks; comet assay
n4:klicoveSlovo
n18:DNA%20crosslinks n18:cisplatin n18:comet%20assay
n4:kodStatuVydavatele
SK - Slovenská republika
n4:kontrolniKodProRIV
[A70C7000F1EF]
n4:nazevZdroje
Neoplasma
n4:obor
n17:FD
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
7
n4:rokUplatneniVysledku
n14:2013
n4:svazekPeriodika
60
n4:tvurceVysledku
Fikrová, Petra Jošt, Petr Hrnčiarik, Michal Řeháček, Vít Hronek, Miloslav Zadák, Zdeněk Štětina, Rudolf
n4:wos
000319535400008
s:issn
0028-2685
s:numberOfPages
6
n8:doi
10.4149/neo_2013_053
n10:organizacniJednotka
11160