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Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F13%3A10144102%21RIV14-GA0-11160___
rdf:type
n5:Vysledek skos:Concept
rdfs:seeAlso
http://www.hindawi.com/journals/bmri/2013/408573/
dcterms:description
Process of aging is accompanied by changes in the biotransformation of xenobiotics and impairment of normal cellular functions by free radicals. Therefore, this study was designed to determine age-related differences in the activities and/or expressions of selected drug-metabolizing and antioxidant enzymes in young and old rats. Specific activities of 8 drug-metabolizing enzymes and 4 antioxidant enzymes were assessed in hepatic subcellular fractions of 6-week-old and 21-month-old male Wistar rats. Protein expressions of carbonyl reductase 1 (CBR1) and glutathione S-transferase (GST) were determined using immunoblotting. Remarkable age-related decrease in specific activities of CYP2B, CYP3A, and UDP-glucuronosyl transferase was observed, whereas no changes in activities of CYP1A2, flavine monooxygenase, aldo-keto reductase 1C, and antioxidant enzymes with advancing age were found. On the other hand, specific activity of CBR1 and GST was 2.4 folds and 5.6 folds higher in the senescent rats compared with the young ones, respectively. Interindividual variability in CBR1 activity increased significantly with rising age. We suppose that elevated activities of GST and CBR1 may protect senescent rats against xenobiotic as well as eobiotic electrophiles and reactive carbonyls, but they may alter metabolism of drugs, which are CBR1 and especially GSTs substrates. Process of aging is accompanied by changes in the biotransformation of xenobiotics and impairment of normal cellular functions by free radicals. Therefore, this study was designed to determine age-related differences in the activities and/or expressions of selected drug-metabolizing and antioxidant enzymes in young and old rats. Specific activities of 8 drug-metabolizing enzymes and 4 antioxidant enzymes were assessed in hepatic subcellular fractions of 6-week-old and 21-month-old male Wistar rats. Protein expressions of carbonyl reductase 1 (CBR1) and glutathione S-transferase (GST) were determined using immunoblotting. Remarkable age-related decrease in specific activities of CYP2B, CYP3A, and UDP-glucuronosyl transferase was observed, whereas no changes in activities of CYP1A2, flavine monooxygenase, aldo-keto reductase 1C, and antioxidant enzymes with advancing age were found. On the other hand, specific activity of CBR1 and GST was 2.4 folds and 5.6 folds higher in the senescent rats compared with the young ones, respectively. Interindividual variability in CBR1 activity increased significantly with rising age. We suppose that elevated activities of GST and CBR1 may protect senescent rats against xenobiotic as well as eobiotic electrophiles and reactive carbonyls, but they may alter metabolism of drugs, which are CBR1 and especially GSTs substrates.
dcterms:title
Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats
skos:prefLabel
Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats Age-Related Changes in Hepatic Activity and Expression of Detoxification Enzymes in Male Rats
skos:notation
RIV/00216208:11160/13:10144102!RIV14-GA0-11160___
n5:predkladatel
n10:orjk%3A11160
n3:aktivita
n4:I n4:S n4:P
n3:aktivity
I, P(GBP303/12/G163), S
n3:cisloPeriodika
July
n3:dodaniDat
n14:2014
n3:domaciTvurceVysledku
n19:4058445 n19:1405764 n19:6001211 n19:2270455 n19:2442256 n19:9958185
n3:druhVysledku
n9:J
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
59738
n3:idVysledku
RIV/00216208:11160/13:10144102
n3:jazykVysledku
n13:eng
n3:klicovaSlova
rat; liver; glutathione S-transferase; carbonyl reductase 1; biotransformation enzymes; antioxidant enzymes; aging
n3:klicoveSlovo
n11:glutathione%20S-transferase n11:biotransformation%20enzymes n11:antioxidant%20enzymes n11:rat n11:liver n11:aging n11:carbonyl%20reductase%201
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[8D6FCBDEFD8B]
n3:nazevZdroje
BioMed Research International
n3:obor
n15:CE
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n20:GBP303%2F12%2FG163
n3:rokUplatneniVysledku
n14:2013
n3:svazekPeriodika
2013
n3:tvurceVysledku
Vyskočilová, Erika Bártíková, Hana Hlaváčová, Jitka Boušová, Iva Szotáková, Barbora Skálová, Lenka
n3:wos
000322656400001
s:issn
2314-6133
s:numberOfPages
10
n17:doi
10.1155/2013/408573
n16:organizacniJednotka
11160