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Statements

Subject Item
n2:RIV%2F00216208%3A11160%2F13%3A10144048%21RIV14-MSM-11160___
rdf:type
skos:Concept n9:Vysledek
rdfs:seeAlso
http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060796
dcterms:description
In this study, four structurally related hydroxyflavones (baicalein, mosloflavone, negletein, and 5,6-dihydroxyflavone) at very low and physiologically relevant levels were tested to distinguish antioxidant activity from cytotoxic effects using two different cell lines, L-6 myoblasts and THP-1 monocytes. Measurements showed strong antioxidant activities for baicalein and 5,6-dihydroxyflavone at picomolar concentrations, while 10 nM partially protected monocytes against the strong oxidative stress induced by cumene hydroperoxide. Analysis of wide range dose-dependence curves characterizing the mechanisms made it possible to exclude a protein-mediated antioxidant response, as well as a mechanism based on the simple stoichiometric scavenging of radicals. The results demonstrated that these flavones do not act on the same radicals as the flavonol quercetin. Considering the normal concentrations of all the endogenous antioxidants in cells, the addition of picomolar or nanomolar levels of these flavones should not be expected to produce any detectable increase in the total cellular antioxidant capacity. The significant intracellular antioxidant activity observed with 1 pM baicalein means that it must be scavenging radicals that for some reason are not eliminated by the endogenous antioxidants. The strong antioxidant effects found suggest these flavones, as well as quercetin and similar polyphenolic antioxidants, at physiologically relevant concentrations act as redox mediators to enable endogenous antioxidants to reach and scavenge different pools of otherwise inaccessible radicals. In this study, four structurally related hydroxyflavones (baicalein, mosloflavone, negletein, and 5,6-dihydroxyflavone) at very low and physiologically relevant levels were tested to distinguish antioxidant activity from cytotoxic effects using two different cell lines, L-6 myoblasts and THP-1 monocytes. Measurements showed strong antioxidant activities for baicalein and 5,6-dihydroxyflavone at picomolar concentrations, while 10 nM partially protected monocytes against the strong oxidative stress induced by cumene hydroperoxide. Analysis of wide range dose-dependence curves characterizing the mechanisms made it possible to exclude a protein-mediated antioxidant response, as well as a mechanism based on the simple stoichiometric scavenging of radicals. The results demonstrated that these flavones do not act on the same radicals as the flavonol quercetin. Considering the normal concentrations of all the endogenous antioxidants in cells, the addition of picomolar or nanomolar levels of these flavones should not be expected to produce any detectable increase in the total cellular antioxidant capacity. The significant intracellular antioxidant activity observed with 1 pM baicalein means that it must be scavenging radicals that for some reason are not eliminated by the endogenous antioxidants. The strong antioxidant effects found suggest these flavones, as well as quercetin and similar polyphenolic antioxidants, at physiologically relevant concentrations act as redox mediators to enable endogenous antioxidants to reach and scavenge different pools of otherwise inaccessible radicals.
dcterms:title
Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism
skos:prefLabel
Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism Protection of Cells against Oxidative Stress by Nanomolar Levels of Hydroxyflavones Indicates a New Type of Intracellular Antioxidant Mechanism
skos:notation
RIV/00216208:11160/13:10144048!RIV14-MSM-11160___
n9:predkladatel
n16:orjk%3A11160
n3:aktivita
n17:I
n3:aktivity
I
n3:cisloPeriodika
4
n3:dodaniDat
n8:2014
n3:domaciTvurceVysledku
n5:4568869 n5:9622314 n5:2446197
n3:druhVysledku
n14:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
100508
n3:idVysledku
RIV/00216208:11160/13:10144048
n3:jazykVysledku
n15:eng
n3:klicovaSlova
inhibition; 2'-hydroxychalcones; wogonin; activation; proliferation; apoptosis; flavonoids; scutellaria; baicalein; vitamin-e
n3:klicoveSlovo
n6:apoptosis n6:wogonin n6:proliferation n6:activation n6:scutellaria n6:baicalein n6:2%27-hydroxychalcones n6:inhibition n6:flavonoids n6:vitamin-e
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[5A8D59F369CC]
n3:nazevZdroje
PLoS ONE
n3:obor
n20:FR
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
16
n3:rokUplatneniVysledku
n8:2013
n3:svazekPeriodika
8
n3:tvurceVysledku
Saso, Luciano Pedersen, Jens Z. Filipský, Tomáš Lombardo, Emanuele Incerpi, Sandra Bavavea, Eugenia I. Hájek, Jan Bovicelli, Paolo Balducci, Valentina Silvestri, Ilaria Proietti Righi, Giuliana Sabellico, Cristian De Vito, Paolo Staňková, Veronika Luly, Paolo Leone, Stefano
n3:wos
000317908700008
s:issn
1932-6203
s:numberOfPages
12
n19:doi
10.1371/journal.pone.0060796
n13:organizacniJednotka
11160