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Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F14%3A10281562%21RIV15-MSM-11150___
rdf:type
skos:Concept n15:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.3233/CH-131743
dcterms:description
Intestinal microcirculatory disturbances play an important role in the pathophysiology of sepsis. A neural anti-inflammatory pathway has been suggested as a potential target for therapy that may dampen systemic inflammation. The aim of this study is to investigate the effects of physostigmine, a cholinesterase inhibitor, on the intestinal microcirculation and vascular contractility in experimental endotoxemia. Endotoxemia was induced in Lewis rats by intravenous lipopolysaccharide (LPS) administration. Animals were treated with either physostigmine or saline (control) following LPS challenge. The intestinal microcirculation, including leukocyte-endothelial interaction, functional capillary density (FCD) and non-perfused capillary density (NCD), was examined by intravital microscopy (IVM) 2 hours after LPS administration. The impact of physostigmine on vascular contractility of rat aortic rings was examined by in vitro myography. Physostigmine significantly reduced the number of adhering leukocytes in intestinal submucosal venules (V1 venules: -61%, V3 venules: -36%) of LPS animals. FCD was significantly increased by physostigmine treatment (circular muscle layer: +180%, longitudinal muscle layer: +162%, mucosa: +149%). Low concentrations of physostigmine produced significant contraction of aortic ring preparations, whereas high concentrations produced relaxation. In conclusion, physostigmine treatment significantly improved the intestinal microcirculation in experimental endotoxemia by reducing leukocyte adhesion and increasing FCD. Intestinal microcirculatory disturbances play an important role in the pathophysiology of sepsis. A neural anti-inflammatory pathway has been suggested as a potential target for therapy that may dampen systemic inflammation. The aim of this study is to investigate the effects of physostigmine, a cholinesterase inhibitor, on the intestinal microcirculation and vascular contractility in experimental endotoxemia. Endotoxemia was induced in Lewis rats by intravenous lipopolysaccharide (LPS) administration. Animals were treated with either physostigmine or saline (control) following LPS challenge. The intestinal microcirculation, including leukocyte-endothelial interaction, functional capillary density (FCD) and non-perfused capillary density (NCD), was examined by intravital microscopy (IVM) 2 hours after LPS administration. The impact of physostigmine on vascular contractility of rat aortic rings was examined by in vitro myography. Physostigmine significantly reduced the number of adhering leukocytes in intestinal submucosal venules (V1 venules: -61%, V3 venules: -36%) of LPS animals. FCD was significantly increased by physostigmine treatment (circular muscle layer: +180%, longitudinal muscle layer: +162%, mucosa: +149%). Low concentrations of physostigmine produced significant contraction of aortic ring preparations, whereas high concentrations produced relaxation. In conclusion, physostigmine treatment significantly improved the intestinal microcirculation in experimental endotoxemia by reducing leukocyte adhesion and increasing FCD.
dcterms:title
Physostigmine reverses disturbances of the intestinal microcirculation during experimental endotoxemia Physostigmine reverses disturbances of the intestinal microcirculation during experimental endotoxemia
skos:prefLabel
Physostigmine reverses disturbances of the intestinal microcirculation during experimental endotoxemia Physostigmine reverses disturbances of the intestinal microcirculation during experimental endotoxemia
skos:notation
RIV/00216208:11150/14:10281562!RIV15-MSM-11150___
n4:aktivita
n13:I
n4:aktivity
I
n4:cisloPeriodika
3
n4:dodaniDat
n7:2015
n4:domaciTvurceVysledku
n12:2920387
n4:druhVysledku
n10:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
36606
n4:idVysledku
RIV/00216208:11150/14:10281562
n4:jazykVysledku
n9:eng
n4:klicovaSlova
intravital microscopy; endotoxemia; sepsis; microcirculation; Physostigmine
n4:klicoveSlovo
n8:microcirculation n8:intravital%20microscopy n8:endotoxemia n8:Physostigmine n8:sepsis
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[D1E8E9D37830]
n4:nazevZdroje
Clinical Hemorheology and Microcirculation
n4:obor
n18:FE
n4:pocetDomacichTvurcuVysledku
1
n4:pocetTvurcuVysledku
12
n4:rokUplatneniVysledku
n7:2014
n4:svazekPeriodika
56
n4:tvurceVysledku
Saleh Abdo, Islam Samir Mohamed Hung, Orlando Whynot, Sara Spassov, Alexander Zhou, Juan Rueb, Johanna Pavlovic, Dragan Kern, Hartmut Černý, Vladimír Lehmann, Christian Masur, Stefan Shukla, Romesh
n4:wos
000334341800009
s:issn
1386-0291
s:numberOfPages
12
n11:doi
10.3233/CH-131743
n17:organizacniJednotka
11150