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Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F13%3A10191974%21RIV14-MSM-11150___
rdf:type
skos:Concept n12:Vysledek
rdfs:seeAlso
http://biomed.papers.upol.cz/pdfs/bio/2013/04/05.pdf
dcterms:description
Background. Epigenetic changes are considered to be a frequent event during tumor development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumor suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in endometrial cancer by comparison with normal endometrial tissue. Materials and Methods. We used MS-MLPA (Methylation-specific Multiplex ligation-dependent probe amplification) to compare the methylation status of 59 tissue samples of endometroid type of endometrial carcinoma with 20 control samples of non-neoplastic endometrium. Results. Using 15% cut-off for methylation, we observed significantly higher methylation in the CDH13 gene in endometrial cancer group. We observed significantly higher methylation in both WT1 and GATA5 genes in IB stage of endometroid carcinoma. We also observed significantly higher methylation in GATA5 gene in the group of poorly differentiated endometroid carcinoma. Conclusion. The findings suggest the importance of hypermethylation of CDH13, WT1 and GATA5 genes in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies of endometrial cancer based on epigenetic changes. Background. Epigenetic changes are considered to be a frequent event during tumor development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumor suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in endometrial cancer by comparison with normal endometrial tissue. Materials and Methods. We used MS-MLPA (Methylation-specific Multiplex ligation-dependent probe amplification) to compare the methylation status of 59 tissue samples of endometroid type of endometrial carcinoma with 20 control samples of non-neoplastic endometrium. Results. Using 15% cut-off for methylation, we observed significantly higher methylation in the CDH13 gene in endometrial cancer group. We observed significantly higher methylation in both WT1 and GATA5 genes in IB stage of endometroid carcinoma. We also observed significantly higher methylation in GATA5 gene in the group of poorly differentiated endometroid carcinoma. Conclusion. The findings suggest the importance of hypermethylation of CDH13, WT1 and GATA5 genes in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies of endometrial cancer based on epigenetic changes.
dcterms:title
Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA
skos:prefLabel
Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA
skos:notation
RIV/00216208:11150/13:10191974!RIV14-MSM-11150___
n12:predkladatel
n15:orjk%3A11150
n3:aktivita
n18:S n18:I
n3:aktivity
I, S
n3:cisloPeriodika
4
n3:dodaniDat
n14:2014
n3:domaciTvurceVysledku
n5:6953891 n5:1117149 n5:3299643 n5:2010887 n5:8934460
n3:druhVysledku
n20:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
87656
n3:idVysledku
RIV/00216208:11150/13:10191974
n3:jazykVysledku
n4:eng
n3:klicovaSlova
epigenetics; GATA5; WT1; CDH13; endometrial cancer; DNA methylation; MS-MLPA
n3:klicoveSlovo
n10:epigenetics n10:endometrial%20cancer n10:DNA%20methylation n10:WT1 n10:GATA5 n10:CDH13 n10:MS-MLPA
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[AF59BD818E4F]
n3:nazevZdroje
Biomedical Papers
n3:obor
n17:FK
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:rokUplatneniVysledku
n14:2013
n3:svazekPeriodika
157
n3:tvurceVysledku
Chmelařová, Marcela Špaček, Jiří Laco, Jan Palička, Vladimír Dvořáková, Eva
n3:wos
000329091500006
s:issn
1213-8118
s:numberOfPages
6
n19:doi
10.5507/bp.2013.035
n13:organizacniJednotka
11150