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Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F13%3A10173671%21RIV14-GA0-11150___
rdf:type
n7:Vysledek skos:Concept
rdfs:seeAlso
http://www.spandidos-publications.com/or/30/6/2593
dcterms:description
In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 µM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of α-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of α-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of α-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in α-tomatine-treated cells. These results support the conclusion that α-tomatine does not induce apoptosis in the MCF-7 cell line. In recent years, α-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of α-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 µM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of α-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of α-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of α-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in α-tomatine-treated cells. These results support the conclusion that α-tomatine does not induce apoptosis in the MCF-7 cell line.
dcterms:title
The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
skos:prefLabel
The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
skos:notation
RIV/00216208:11150/13:10173671!RIV14-GA0-11150___
n7:predkladatel
n10:orjk%3A11150
n3:aktivita
n20:I n20:S n20:P
n3:aktivity
I, P(EE2.3.30.0022), P(GPP303/12/P536), S
n3:cisloPeriodika
6
n3:dodaniDat
n12:2014
n3:domaciTvurceVysledku
n4:5403065 n4:2975858 n4:6518672 n4:2441926 n4:9917829 n4:8982007 n4:1537989 n4:7723830 n4:5046572 n4:2650673
n3:druhVysledku
n21:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n6:predkladatel
n3:idSjednocenehoVysledku
67674
n3:idVysledku
RIV/00216208:11150/13:10173671
n3:jazykVysledku
n17:eng
n3:klicovaSlova
cholesterol; cell death; MCF-7; alpha-tomatine
n3:klicoveSlovo
n13:alpha-tomatine n13:MCF-7 n13:cell%20death n13:cholesterol
n3:kodStatuVydavatele
GR - Řecká republika
n3:kontrolniKodProRIV
[53479F992835]
n3:nazevZdroje
Oncology Reports
n3:obor
n5:EB
n3:pocetDomacichTvurcuVysledku
10
n3:pocetTvurcuVysledku
10
n3:projekt
n15:EE2.3.30.0022 n15:GPP303%2F12%2FP536
n3:rokUplatneniVysledku
n12:2013
n3:svazekPeriodika
30
n3:tvurceVysledku
Řezáčová, Martina Bezrouk, Aleš Tomšík, Pavel Ćmielová, Jana Rudolf, Emil Köhlerová, Renata Hroch, Miloš Suchá, Lenka Šišpera, Luděk Havelek, Radim
n3:wos
000330226200007
s:issn
1021-335X
s:numberOfPages
10
n18:doi
10.3892/or.2013.2778
n16:organizacniJednotka
11150