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Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F13%3A10138893%21RIV14-MSM-11150___
rdf:type
skos:Concept n5:Vysledek
rdfs:seeAlso
http://www.jpp.krakow.pl/journal/archive/04_13/articles/09_article.html
dcterms:description
The multimarker approach using Luminex technology represents a new tool for studying the pathogenesis of cardiovascular disease. Although many cardiac biomarkers in heart failure have been well established, the role and significance of their measurement in hypertensive patients is still questionable. The aim of our study was to evaluate the relationship of selected biomarkers in L-NAME-induced hypertension to the left ventricular remodeling in the two different periods of hypertension development. Four groups of 3-month-old male Wistar rats were investigated: (1) control 4 (placebo for 4 weeks), (2) control 7 (placebo for 7 weeks), (3) L-NAME 4 (40 mg/kg/day for 4 weeks), and (4) L-NAME 7 (40 mg/kg/day for 7 weeks). BNP, cTnI, TNF-alpha, and VEGF were measured using Rat CVD Panel 1 Kit (Milliplex (R) MAP). Cardiac troponin T was determined using Elecsys (R) Troponin T high sensitive immunoassay (Roche, Switzerland). Although the systolic blood pressure increases about 50% in L-NAME-induced hypertension in rat, both hypertrophy and fibrosis were expressed only slightly in this experiment. The levels of BNP, TNF-alpha, or VEGF did not differ significantly among groups. However, cardiac troponin T measured by high sensitive ELISA was significantly (P<0.05) increased in L-NAME 4 (0.229 mu g/l versus 0.034 mu g/l) and L-NAME-7 groups (0.366 mu g/l versus 0.06 mu g/l) in comparison with the controls. We conclude that the slightly increased cTnT levels could indicate ischemic damage of L-NAME-hypertensive heart. Importantly, to our best knowledge, this is the first study indicating that CVD rat panel may be a useful methodological tool in experimental cardiology. The multimarker approach using Luminex technology represents a new tool for studying the pathogenesis of cardiovascular disease. Although many cardiac biomarkers in heart failure have been well established, the role and significance of their measurement in hypertensive patients is still questionable. The aim of our study was to evaluate the relationship of selected biomarkers in L-NAME-induced hypertension to the left ventricular remodeling in the two different periods of hypertension development. Four groups of 3-month-old male Wistar rats were investigated: (1) control 4 (placebo for 4 weeks), (2) control 7 (placebo for 7 weeks), (3) L-NAME 4 (40 mg/kg/day for 4 weeks), and (4) L-NAME 7 (40 mg/kg/day for 7 weeks). BNP, cTnI, TNF-alpha, and VEGF were measured using Rat CVD Panel 1 Kit (Milliplex (R) MAP). Cardiac troponin T was determined using Elecsys (R) Troponin T high sensitive immunoassay (Roche, Switzerland). Although the systolic blood pressure increases about 50% in L-NAME-induced hypertension in rat, both hypertrophy and fibrosis were expressed only slightly in this experiment. The levels of BNP, TNF-alpha, or VEGF did not differ significantly among groups. However, cardiac troponin T measured by high sensitive ELISA was significantly (P<0.05) increased in L-NAME 4 (0.229 mu g/l versus 0.034 mu g/l) and L-NAME-7 groups (0.366 mu g/l versus 0.06 mu g/l) in comparison with the controls. We conclude that the slightly increased cTnT levels could indicate ischemic damage of L-NAME-hypertensive heart. Importantly, to our best knowledge, this is the first study indicating that CVD rat panel may be a useful methodological tool in experimental cardiology.
dcterms:title
Multiplexed immunoassays for simultaneous quantification of cardiovascular biomarkers in the model of HG-nitro-L-arginine methylester (L-name) hypertensive rat Multiplexed immunoassays for simultaneous quantification of cardiovascular biomarkers in the model of HG-nitro-L-arginine methylester (L-name) hypertensive rat
skos:prefLabel
Multiplexed immunoassays for simultaneous quantification of cardiovascular biomarkers in the model of HG-nitro-L-arginine methylester (L-name) hypertensive rat Multiplexed immunoassays for simultaneous quantification of cardiovascular biomarkers in the model of HG-nitro-L-arginine methylester (L-name) hypertensive rat
skos:notation
RIV/00216208:11150/13:10138893!RIV14-MSM-11150___
n5:predkladatel
n6:orjk%3A11150
n7:aktivita
n19:I n19:Z
n7:aktivity
I, Z(AV0Z50520701)
n7:cisloPeriodika
2
n7:dodaniDat
n8:2014
n7:domaciTvurceVysledku
n14:7344856
n7:druhVysledku
n18:J
n7:duvernostUdaju
n10:S
n7:entitaPredkladatele
n11:predkladatel
n7:idSjednocenehoVysledku
90154
n7:idVysledku
RIV/00216208:11150/13:10138893
n7:jazykVysledku
n16:eng
n7:klicovaSlova
myocardial hypertrophy; heart remodeling; hypertension; tumor necrosis factor-alpha; vascular endothelial growth factor; beta-natriuretic peptide; cardiac troponin; multiplex assay; cardiac biomarkers
n7:klicoveSlovo
n12:multiplex%20assay n12:hypertension n12:heart%20remodeling n12:cardiac%20troponin n12:vascular%20endothelial%20growth%20factor n12:cardiac%20biomarkers n12:tumor%20necrosis%20factor-alpha n12:beta-natriuretic%20peptide n12:myocardial%20hypertrophy
n7:kodStatuVydavatele
PL - Polská republika
n7:kontrolniKodProRIV
[E143B6A29B1B]
n7:nazevZdroje
Journal of Physiology and Pharmacology
n7:obor
n13:ED
n7:pocetDomacichTvurcuVysledku
1
n7:pocetTvurcuVysledku
3
n7:rokUplatneniVysledku
n8:2013
n7:svazekPeriodika
64
n7:tvurceVysledku
Adamcová, Michaela Šimko, Fedor Růžičková, Šárka
n7:wos
000320830100009
n7:zamer
n15:AV0Z50520701
s:issn
0867-5910
s:numberOfPages
7
n17:organizacniJednotka
11150