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Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F13%3A10130535%21RIV14-MSM-11150___
rdf:type
n6:Vysledek skos:Concept
rdfs:seeAlso
http://www.degruyter.com/view/j/jab.2013.11.issue-2/v10136-012-0033-8/v10136-012-0033-8.xml
dcterms:description
Alpha-tomatine is a major glycoalkaloid found in the roots, leaves, stems and fruit of tomatoes Lycopersicon esculentum. Recently, alpha-tomatine has been recognized as a potential anticancer drug. In the present study, we identified the signaling cascades involved in the antitumor effect of alpha-tomatine on MOLT-4 leukemic cells. Alpha-tomatine inhibited the proliferation and decreased the viability of MOLT-4 cells in a dose-dependent manner. An increase in the activity of caspases 9 and 3/7 was not observed. However, an increase in the amount of p53 and its phosphorylation on serine 15, as well as an increased amount of mitochondrial protein PUMA was detected 4 and 24 h after exposure to alpha-tomatine at a concentration of 1-3 μmol/l. Inhibition of the proliferation of MOLT-4 cells by alpha-tomatine is also associated with an increase in p21WAF1/CIP1 and the activation of Chk2. The comet assay did not detect significant amounts of single or double DNA strand breaks in cells treated with alpha-tomatine at concentrations of 0.1-9 μmol/l. Our results thus contribute to the understanding of the anticancer action of alpha-tomatine. Alpha-tomatine is a major glycoalkaloid found in the roots, leaves, stems and fruit of tomatoes Lycopersicon esculentum. Recently, alpha-tomatine has been recognized as a potential anticancer drug. In the present study, we identified the signaling cascades involved in the antitumor effect of alpha-tomatine on MOLT-4 leukemic cells. Alpha-tomatine inhibited the proliferation and decreased the viability of MOLT-4 cells in a dose-dependent manner. An increase in the activity of caspases 9 and 3/7 was not observed. However, an increase in the amount of p53 and its phosphorylation on serine 15, as well as an increased amount of mitochondrial protein PUMA was detected 4 and 24 h after exposure to alpha-tomatine at a concentration of 1-3 μmol/l. Inhibition of the proliferation of MOLT-4 cells by alpha-tomatine is also associated with an increase in p21WAF1/CIP1 and the activation of Chk2. The comet assay did not detect significant amounts of single or double DNA strand breaks in cells treated with alpha-tomatine at concentrations of 0.1-9 μmol/l. Our results thus contribute to the understanding of the anticancer action of alpha-tomatine.
dcterms:title
Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells
skos:prefLabel
Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells
skos:notation
RIV/00216208:11150/13:10130535!RIV14-MSM-11150___
n6:predkladatel
n11:orjk%3A11150
n3:aktivita
n4:I
n3:aktivity
I
n3:cisloPeriodika
2
n3:dodaniDat
n15:2014
n3:domaciTvurceVysledku
n5:6518672 n5:6452965 n5:1537989 n5:3523322 n5:5046572 n5:8982007
n3:druhVysledku
n8:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
60150
n3:idVysledku
RIV/00216208:11150/13:10130535
n3:jazykVysledku
n7:eng
n3:klicovaSlova
leukemia; PUMA; p53; DNA damage; alpha-tomatine
n3:klicoveSlovo
n12:p53 n12:leukemia n12:DNA%20damage n12:PUMA n12:alpha-tomatine
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[F065F4AF111B]
n3:nazevZdroje
Journal of Applied Biomedicine
n3:obor
n20:FR
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n15:2013
n3:svazekPeriodika
11
n3:tvurceVysledku
Tomšík, Pavel Suchá, Lenka Havelek, Radim Seifrtová, Martina Řezáčová, Martina Kúdelová, Jana
n3:wos
000314809600005
s:issn
1214-021X
s:numberOfPages
11
n18:doi
10.2478/v10136-012-0033-8
n14:organizacniJednotka
11150