This HTML5 document contains 50 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
n19http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216208%3A11150%2F12%3A10125002%21RIV13-MSM-11150___/
dctermshttp://purl.org/dc/terms/
n16http://localhost/temp/predkladatel/
n11http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n7http://linked.opendata.cz/resource/domain/vavai/subjekt/
n6http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n3http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n8http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n18http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n15http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n9http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n13http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n4http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F12%3A10125002%21RIV13-MSM-11150___
rdf:type
n6:Vysledek skos:Concept
rdfs:seeAlso
http://www.biomed.cas.cz/physiolres/pdf/61%20Suppl%202/61_S77.pdf
dcterms:description
Decades of liver regeneration studies still left the termination phase least elucidated. However regeneration ending mechanisms are clinically relevant. We aimed to analyse the timing and transcriptional control of the latest phase of liver regeneration, both controversial. Male Wistar rats were subjected to 2/3 partial hepatectomy with recovery lasting from 1 to 14 days. Time-series microarray data were assessed by innovative combination of hierarchical clustering and principal component analysis and validated by real-time RT-PCR. Hierarchical clustering and principal component analysis in agreement distinguished three temporal phases of liver regeneration. We found 359 genes specifically altered during late phase regeneration. Gene enrichment analysis and manual review of microarray data suggested five pathways worth further study: PPAR signalling pathway; lipid metabolism; complement, coagulation and fibrinolytic cascades; ECM remodelling and xenobiotic biotransformation. Microarray findings pertinent for termination phase were substantiated by real-time RT-PCR. In conclusion, transcriptional profiling mapped late phase of liver regeneration beyond 5th day of recovery and revealed 5 pathways specifically acting at this time. Inclusion of longer post-surgery intervals brought improved coverage of regeneration time dynamics and is advisable for further works. Investigation into the workings of suggested pathways might prove helpful in preventing and managing liver tumours. Decades of liver regeneration studies still left the termination phase least elucidated. However regeneration ending mechanisms are clinically relevant. We aimed to analyse the timing and transcriptional control of the latest phase of liver regeneration, both controversial. Male Wistar rats were subjected to 2/3 partial hepatectomy with recovery lasting from 1 to 14 days. Time-series microarray data were assessed by innovative combination of hierarchical clustering and principal component analysis and validated by real-time RT-PCR. Hierarchical clustering and principal component analysis in agreement distinguished three temporal phases of liver regeneration. We found 359 genes specifically altered during late phase regeneration. Gene enrichment analysis and manual review of microarray data suggested five pathways worth further study: PPAR signalling pathway; lipid metabolism; complement, coagulation and fibrinolytic cascades; ECM remodelling and xenobiotic biotransformation. Microarray findings pertinent for termination phase were substantiated by real-time RT-PCR. In conclusion, transcriptional profiling mapped late phase of liver regeneration beyond 5th day of recovery and revealed 5 pathways specifically acting at this time. Inclusion of longer post-surgery intervals brought improved coverage of regeneration time dynamics and is advisable for further works. Investigation into the workings of suggested pathways might prove helpful in preventing and managing liver tumours.
dcterms:title
Transcriptome Temporal and Functional Analysis of Liver Regeneration Termination Transcriptome Temporal and Functional Analysis of Liver Regeneration Termination
skos:prefLabel
Transcriptome Temporal and Functional Analysis of Liver Regeneration Termination Transcriptome Temporal and Functional Analysis of Liver Regeneration Termination
skos:notation
RIV/00216208:11150/12:10125002!RIV13-MSM-11150___
n6:predkladatel
n7:orjk%3A11150
n3:aktivita
n9:S n9:I
n3:aktivity
I, S
n3:cisloPeriodika
Suppl. 2
n3:dodaniDat
n4:2013
n3:domaciTvurceVysledku
n11:9854185 n11:2132737 n11:2009811 n11:6653227
n3:druhVysledku
n10:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
174856
n3:idVysledku
RIV/00216208:11150/12:10125002
n3:jazykVysledku
n15:eng
n3:klicovaSlova
rat; termination; gene expression profiling; hepatectomy; Liver regeneration
n3:klicoveSlovo
n8:Liver%20regeneration n8:hepatectomy n8:gene%20expression%20profiling n8:rat n8:termination
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[BA5680BF99E1]
n3:nazevZdroje
Physiological Research
n3:obor
n13:ED
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n4:2012
n3:svazekPeriodika
61
n3:tvurceVysledku
Bunček, Martin Libra, Antonín Rychtrmoc, David Víšková, Alena Červinková, Zuzana Hubálková, Lenka
n3:wos
000310260000010
s:issn
0862-8408
s:numberOfPages
16
n16:organizacniJednotka
11150