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Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F07%3A00004289%21RIV07-MSM-11150___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Jaterní hvězdicové buňky (HSC) a jaterní myofibroblasty (MFB) jsou dvě buněčné populace pravděpodobně zodpovědné za syntézu většiny vaziva ve fibrotických játrech. Z normálních jater potkanů i z jater cirhotických jsme izolovali HSC perfuzí roztoky pronasy a kolagenasy. Suspensi buněk jsme dělili centrifugací na hustotním gradientu. HSC byly klidové po 2 dnech kultivace ale dalších 5 dnů vedlo k jejich aktivaci. Pasážováním buněk se značné změnil jejich charakter, HSC byly nahrazeny myofibroblasty. Exprese 49 genů kódujících vazivové bílkoviny, proteoglykany, metalloproteasy a jejich inhibitory a cytokiny byla stanovena s použitím DNA mikroarrayí, a to v klidových HSC, v aktivovaných HSC a v myofibroblastech. Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. Normal and carbon tetrachloride-cirrhotic livers from rats were used to prepare HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined in quiescent HSC, activated HSC and MFB. Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. Normal and carbon tetrachloride-cirrhotic livers from rats were used to prepare HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined in quiescent HSC, activated HSC and MFB.
dcterms:title
Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts Exprese of mRNA, které mají vztah k metabolismu pojivové tkáně, v jaterních hvězdicových buňkách a myofibroblastech
skos:prefLabel
Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts Exprese of mRNA, které mají vztah k metabolismu pojivové tkáně, v jaterních hvězdicových buňkách a myofibroblastech Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts
skos:notation
RIV/00216208:11150/07:00004289!RIV07-MSM-11150___
n3:strany
263;273
n3:aktivita
n13:Z n13:P
n3:aktivity
P(GA305/03/1513), Z(MO0FVZ0000502), Z(MSM0021620820)
n3:cisloPeriodika
4
n3:dodaniDat
n18:2007
n3:domaciTvurceVysledku
n8:9078150 n8:7845685 n8:6849415 n8:7044437 n8:4471113
n3:druhVysledku
n17:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
421416
n3:idVysledku
RIV/00216208:11150/07:00004289
n3:jazykVysledku
n5:eng
n3:klicovaSlova
hepatic stellate cells; liver myofibroblasts; gene expression; connective tissue; liver cirrhosis
n3:klicoveSlovo
n4:hepatic%20stellate%20cells n4:liver%20myofibroblasts n4:liver%20cirrhosis n4:connective%20tissue n4:gene%20expression
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[268A432A3050]
n3:nazevZdroje
Experimental and Toxicological Pathology
n3:obor
n19:FP
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
10
n3:projekt
n11:GA305%2F03%2F1513
n3:rokUplatneniVysledku
n18:2007
n3:svazekPeriodika
58
n3:tvurceVysledku
Jiroutová, Alena Kanta, Jiří Bolehovská, Radka Hajzlerová, Milena Hanovcová, Irena
n3:zamer
n9:MO0FVZ0000502 n9:MSM0021620820
s:issn
0940-2993
s:numberOfPages
11
n12:organizacniJednotka
11150