This HTML5 document contains 45 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n5http://localhost/temp/predkladatel/
n13http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n15http://linked.opendata.cz/ontology/domain/vavai/
n7http://linked.opendata.cz/resource/domain/vavai/zamer/
n12http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216208%3A11150%2F05%3A00003605%21RIV06-MSM-11150___/
shttp://schema.org/
n6http://linked.opendata.cz/ontology/domain/vavai/riv/
skoshttp://www.w3.org/2004/02/skos/core#
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n14http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n8http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n16http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n11http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216208%3A11150%2F05%3A00003605%21RIV06-MSM-11150___
rdf:type
skos:Concept n15:Vysledek
dcterms:description
Apoptóza indukovaná síranem zinečnatým na buněčné linii HEP-2 byla sledována během 96hodinové expozice. Během prvních 48 hodin zinek vstupoval do mitochondrií a vyvolával tvorbu kyslíkových radikálů. U exponovaných buněk docházelo k mírné aktivaci p53, snížení GSH a poškození mitochondrálních funkcí. Aktivace apoptózy byla charakterizována uvolněním cytochromu C a AIF do cytoplazmy a jádra. Použití různých farmakologických inhibitorů apoptózy (antioxidanty NAC a koenzym Q, kaspázové inhibitory z-DEVD-fmk a z-VAD-fmk, cyklosporin A a bonkgreková kyselina) prokázalo, že zinek účinkuje přímo i nepřímo na mitochondrie a výsledná apoptóza je na kaspázách závislá i nezávislá. Induction of apoptosis by zinc sulfate was investigated during 96 h exposure on the cancer HEP-2 cell line. During 48 h of exposure, zinc translocated into mitochondria and stimulated production of reactive oxygen species (ROS), affected cellular GSH management and induced moderate activation of p53 and dissipation of mitochondrial membrane potential. In Zn-exposed cells, mitochondria released cytochrome c and AIF, whose translocation to the cytoplasm or the nucleus coincided with the activation of apoptosis. The use of various pharmacological inhibitors inhibiting particular apoptotic targets (antioxidants such as N-acetyl-cysteine and coenzyme Q, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk, cyclosporin A and bonkgrekic acid) proved that Zn acts both directly and indirectly on mitochondria and observed apoptosis is executed by caspase-dependent and caspase-independent pathways. Induction of apoptosis by zinc sulfate was investigated during 96 h exposure on the cancer HEP-2 cell line. During 48 h of exposure, zinc translocated into mitochondria and stimulated production of reactive oxygen species (ROS), affected cellular GSH management and induced moderate activation of p53 and dissipation of mitochondrial membrane potential. In Zn-exposed cells, mitochondria released cytochrome c and AIF, whose translocation to the cytoplasm or the nucleus coincided with the activation of apoptosis. The use of various pharmacological inhibitors inhibiting particular apoptotic targets (antioxidants such as N-acetyl-cysteine and coenzyme Q, the caspase inhibitors z-DEVD-fmk and z-VAD-fmk, cyclosporin A and bonkgrekic acid) proved that Zn acts both directly and indirectly on mitochondria and observed apoptosis is executed by caspase-dependent and caspase-independent pathways.
dcterms:title
Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria Zinkem indukovaná apoptóza u HEP-2 nádorových buněk: úloha oxidačního stresu a mitochondrií Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria
skos:prefLabel
Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria Zinc induced apoptosis in HEP-2 cancer cells: The role of oxidative stress and mitochondria Zinkem indukovaná apoptóza u HEP-2 nádorových buněk: úloha oxidačního stresu a mitochondrií
skos:notation
RIV/00216208:11150/05:00003605!RIV06-MSM-11150___
n6:strany
107;120
n6:aktivita
n10:Z
n6:aktivity
Z(MSM0021620820)
n6:cisloPeriodika
2
n6:dodaniDat
n11:2006
n6:domaciTvurceVysledku
n13:2650673 n13:1249746
n6:druhVysledku
n17:J
n6:duvernostUdaju
n8:S
n6:entitaPredkladatele
n12:predkladatel
n6:idSjednocenehoVysledku
552126
n6:idVysledku
RIV/00216208:11150/05:00003605
n6:jazykVysledku
n18:eng
n6:klicovaSlova
Zinc; oxidative stress; energy metabolism; mitochondria; apoptosis
n6:klicoveSlovo
n14:Zinc n14:energy%20metabolism n14:apoptosis n14:oxidative%20stress n14:mitochondria
n6:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n6:kontrolniKodProRIV
[94D844B69D7E]
n6:nazevZdroje
BioFactors
n6:obor
n16:EB
n6:pocetDomacichTvurcuVysledku
2
n6:pocetTvurcuVysledku
3
n6:rokUplatneniVysledku
n11:2005
n6:svazekPeriodika
23
n6:tvurceVysledku
Rudolf, Emil Červinka, Miroslav
n6:zamer
n7:MSM0021620820
s:issn
0951-6433
s:numberOfPages
14
n5:organizacniJednotka
11150