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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F14%3A10293080%21RIV15-MSM-11130___
rdf:type
skos:Concept n18:Vysledek
rdfs:seeAlso
http://dx.doi.org/10.1002/cbf.3056
dcterms:description
Adipose tissue is an abundant source of autologous adult stem cells that may bring new therapeutic perspectives on the treatment of diabetes and its complications. It is unclear whether adipose tissue-derived stromal cells (ASCs) of diabetic patients, constantly influenced by hyperglycaemia, have the same properties as non-diabetic controls. As an alternative source of ASCs, adipose tissue from distal limbs of diabetic patients with critical ischemia was isolated. ASCs were characterized in terms of cell surface markers, multilineage differentiation and the expression of vascular endothelial growth factor (VEGFA), chemokine-related genes and compared with non-diabetic controls. Flow cytometry analysis confirmed mesenchymal phenotypes in both diabetic and non-diabetic ASCs. Nevertheless, 40% of diabetic and 20% of non-diabetic ASC samples displayed high expressions of fibroblast marker, which inversely correlated with the expression of CD105. In diabetic patients, significantly decreased expression of VEGFA and chemokine receptor CXCR4 was found in fibroblast-positive ASCs, compared with their fibroblast-negative counterparts. Reduced osteogenic differentiation and the downregulation of chemokine CXCL12 were found in fibroblast-negative diabetic ASCs. Both diabetic and non-diabetic ASCs were differentiated into adipocytes and chondrocytes and did not reveal islet-like cell differentiation. According to this study, adipose tissue from distal limbs of diabetic patients is not satisfactory as an autologous ASC source. Adipose tissue is an abundant source of autologous adult stem cells that may bring new therapeutic perspectives on the treatment of diabetes and its complications. It is unclear whether adipose tissue-derived stromal cells (ASCs) of diabetic patients, constantly influenced by hyperglycaemia, have the same properties as non-diabetic controls. As an alternative source of ASCs, adipose tissue from distal limbs of diabetic patients with critical ischemia was isolated. ASCs were characterized in terms of cell surface markers, multilineage differentiation and the expression of vascular endothelial growth factor (VEGFA), chemokine-related genes and compared with non-diabetic controls. Flow cytometry analysis confirmed mesenchymal phenotypes in both diabetic and non-diabetic ASCs. Nevertheless, 40% of diabetic and 20% of non-diabetic ASC samples displayed high expressions of fibroblast marker, which inversely correlated with the expression of CD105. In diabetic patients, significantly decreased expression of VEGFA and chemokine receptor CXCR4 was found in fibroblast-positive ASCs, compared with their fibroblast-negative counterparts. Reduced osteogenic differentiation and the downregulation of chemokine CXCL12 were found in fibroblast-negative diabetic ASCs. Both diabetic and non-diabetic ASCs were differentiated into adipocytes and chondrocytes and did not reveal islet-like cell differentiation. According to this study, adipose tissue from distal limbs of diabetic patients is not satisfactory as an autologous ASC source.
dcterms:title
Characterization of human adipose tissue-derived stromal cells isolated from diabetic patient's distal limbs with critical ischemia Characterization of human adipose tissue-derived stromal cells isolated from diabetic patient's distal limbs with critical ischemia
skos:prefLabel
Characterization of human adipose tissue-derived stromal cells isolated from diabetic patient's distal limbs with critical ischemia Characterization of human adipose tissue-derived stromal cells isolated from diabetic patient's distal limbs with critical ischemia
skos:notation
RIV/00216208:11130/14:10293080!RIV15-MSM-11130___
n3:aktivita
n15:I n15:P
n3:aktivity
I, P(ED1.1.00/02.0109), P(GA14-03540S), P(GAP304/11/0653), P(LO1309)
n3:cisloPeriodika
7
n3:dodaniDat
n17:2015
n3:domaciTvurceVysledku
n16:6738109 n16:9894918
n3:druhVysledku
n13:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n20:predkladatel
n3:idSjednocenehoVysledku
6945
n3:idVysledku
RIV/00216208:11130/14:10293080
n3:jazykVysledku
n10:eng
n3:klicovaSlova
mesenchymal stem cells; flow cytometry; differentiation; diabetes; cell therapy; adipose tissue
n3:klicoveSlovo
n4:diabetes n4:cell%20therapy n4:mesenchymal%20stem%20cells n4:adipose%20tissue n4:flow%20cytometry n4:differentiation
n3:kodStatuVydavatele
DK - Dánské království
n3:kontrolniKodProRIV
[CBE0749A0C5E]
n3:nazevZdroje
Cell Biochemistry and Function
n3:obor
n9:FH
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
8
n3:projekt
n7:GAP304%2F11%2F0653 n7:LO1309 n7:ED1.1.00%2F02.0109 n7:GA14-03540S
n3:rokUplatneniVysledku
n17:2014
n3:svazekPeriodika
32
n3:tvurceVysledku
Chudickova, Milada Kubinova, Sarka Baranovicova, Lenka Dubsky, Michal Holan, Vladimir Syková, Eva Kočí, Zuzana Turnovcova, Karolina
n3:wos
000343798900007
s:issn
0263-6484
s:numberOfPages
8
n14:doi
10.1002/cbf.3056
n12:organizacniJednotka
11130