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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F14%3A10293025%21RIV15-MSM-11130___
rdf:type
n10:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1111/pai.12119
dcterms:description
Background Allergen-specific immunotherapy (IT) is widely used to treat allergic diseases. The molecular mechanisms have not been clarified yet completely. The present work was undertaken to analyze the effect of IT in the activation of NF-kappa B. Methods Neutrophils from 15 pollen-allergic IT-treated patients, 10 untreated pollen-allergic patients, and 10 healthy donors were in vitro stimulated with LPS. NF-kappa B activation (p65/p52) was measured in their nuclear extracts by enzyme-linked immunosorbent assay (ELISA). I kappa B alpha phosphorylation, NF-kappa B-repressing factor (NRF) activation, and thromboxane A(2) (TXA(2)) and Interleukin-8 (IL-8) release were measured by ELISA. Results There was a positive correlation between the score of symptoms and NF-kappa B activation in human neutrophils. IT significantly decreased NF-kappa B activation levels in neutrophils compared with neutrophils from untreated patients. I kappa B alpha phosphorylation and NRF activation levels were, respectively, significantly lower and higher in neutrophils from IT-treated patients than from untreated patients. IL-8 and TXA(2) release were significantly lower in neutrophils from IT-treated patients than from untreated patients. Conclusions IT positive effects are at least in part mediated by the negative regulation of NF-kappa B activation in human neutrophils. These observations represent a novel view of neutrophils as possible cell target to treat IgE-dependent diseases through NF-kappa B downmodulation. Background Allergen-specific immunotherapy (IT) is widely used to treat allergic diseases. The molecular mechanisms have not been clarified yet completely. The present work was undertaken to analyze the effect of IT in the activation of NF-kappa B. Methods Neutrophils from 15 pollen-allergic IT-treated patients, 10 untreated pollen-allergic patients, and 10 healthy donors were in vitro stimulated with LPS. NF-kappa B activation (p65/p52) was measured in their nuclear extracts by enzyme-linked immunosorbent assay (ELISA). I kappa B alpha phosphorylation, NF-kappa B-repressing factor (NRF) activation, and thromboxane A(2) (TXA(2)) and Interleukin-8 (IL-8) release were measured by ELISA. Results There was a positive correlation between the score of symptoms and NF-kappa B activation in human neutrophils. IT significantly decreased NF-kappa B activation levels in neutrophils compared with neutrophils from untreated patients. I kappa B alpha phosphorylation and NRF activation levels were, respectively, significantly lower and higher in neutrophils from IT-treated patients than from untreated patients. IL-8 and TXA(2) release were significantly lower in neutrophils from IT-treated patients than from untreated patients. Conclusions IT positive effects are at least in part mediated by the negative regulation of NF-kappa B activation in human neutrophils. These observations represent a novel view of neutrophils as possible cell target to treat IgE-dependent diseases through NF-kappa B downmodulation.
dcterms:title
Structural changes in the bronchial mucosa of young children at risk of developing asthma Structural changes in the bronchial mucosa of young children at risk of developing asthma
skos:prefLabel
Structural changes in the bronchial mucosa of young children at risk of developing asthma Structural changes in the bronchial mucosa of young children at risk of developing asthma
skos:notation
RIV/00216208:11130/14:10293025!RIV15-MSM-11130___
n3:aktivita
n14:S n14:I
n3:aktivity
I, S
n3:cisloPeriodika
2
n3:dodaniDat
n4:2015
n3:domaciTvurceVysledku
n12:9506683 n12:6848249 n12:9066918 n12:7689616
n3:druhVysledku
n16:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
47843
n3:idVysledku
RIV/00216208:11130/14:10293025
n3:jazykVysledku
n5:eng
n3:klicovaSlova
allergen immunotherapy; asthma; neutrophils; NF-kappa B
n3:klicoveSlovo
n13:asthma n13:neutrophils n13:allergen%20immunotherapy n13:NF-kappa%20B
n3:kodStatuVydavatele
DK - Dánské království
n3:kontrolniKodProRIV
[03936420EF61]
n3:nazevZdroje
Pediatric Allergy and Immunology
n3:obor
n18:FG
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n4:2014
n3:svazekPeriodika
25
n3:tvurceVysledku
Beránková, Katarína Hoňková, Lenka Pohunek, Petr Uhlík, Jiří
n3:wos
000332968000005
s:issn
0905-6157
s:numberOfPages
7
n8:doi
10.1111/pai.12119
n9:organizacniJednotka
11130