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Statements

Subject Item
n2:RIV%2F00216208%3A11130%2F13%3A10209663%21RIV14-GA0-11130___
rdf:type
n14:Vysledek skos:Concept
rdfs:seeAlso
http://dx.doi.org/10.1089/scd.2012.0624
dcterms:description
Human embryonic stem cell-derived neural precursors (hESC NPs) are considered to be a promising tool for cell-based therapy in central nervous system injuries and neurodegenerative diseases. The Ca2+ ion is an important intracellular messenger essential for the regulation of various cellular functions. We investigated the role and physiology of Ca2+ signaling to characterize the functional properties of CCTL14 hESC NPs during long-term maintenance in culture (in vitro). We analyzed changes in cytoplasmic Ca2+ concentration ([Ca2+](i)) evoked by high K+, adenosine-5'-triphosphate (ATP), glutamate, gamma-aminobutyric acid (GABA), and caffeine in correlation with the expression of various neuronal markers in different passages (P6 through P10) during the course of hESC differentiation. We found that only differentiated NPs from P7 exhibited significant and specific [Ca2+](i) responses to various stimuli. About 31% of neuronal-like P7 NPs exhibited spontaneous [Ca2+](i) oscillations. Pharmacological and immunocytochemical assays revealed that P7 NPs express L-and P/Q-type Ca2+ channels, P2X(2), P2X(3), P2X(7), and P2Y purinoreceptors, glutamate receptors, and ryanodine (RyR1 and RyR3) receptors. The ATP- and glutamate-induced [Ca2+](i) responses were concentration-dependent. Higher glutamate concentrations (over 100 mu M) caused cell death. Responses to ATP were observed in the presence or in the absence of extracellular Ca2+. These results emphasize the notion that with time in culture, these cells attain a transient period of operative Ca2+ signaling that is predictive of their ability to act as stem elements. Human embryonic stem cell-derived neural precursors (hESC NPs) are considered to be a promising tool for cell-based therapy in central nervous system injuries and neurodegenerative diseases. The Ca2+ ion is an important intracellular messenger essential for the regulation of various cellular functions. We investigated the role and physiology of Ca2+ signaling to characterize the functional properties of CCTL14 hESC NPs during long-term maintenance in culture (in vitro). We analyzed changes in cytoplasmic Ca2+ concentration ([Ca2+](i)) evoked by high K+, adenosine-5'-triphosphate (ATP), glutamate, gamma-aminobutyric acid (GABA), and caffeine in correlation with the expression of various neuronal markers in different passages (P6 through P10) during the course of hESC differentiation. We found that only differentiated NPs from P7 exhibited significant and specific [Ca2+](i) responses to various stimuli. About 31% of neuronal-like P7 NPs exhibited spontaneous [Ca2+](i) oscillations. Pharmacological and immunocytochemical assays revealed that P7 NPs express L-and P/Q-type Ca2+ channels, P2X(2), P2X(3), P2X(7), and P2Y purinoreceptors, glutamate receptors, and ryanodine (RyR1 and RyR3) receptors. The ATP- and glutamate-induced [Ca2+](i) responses were concentration-dependent. Higher glutamate concentrations (over 100 mu M) caused cell death. Responses to ATP were observed in the presence or in the absence of extracellular Ca2+. These results emphasize the notion that with time in culture, these cells attain a transient period of operative Ca2+ signaling that is predictive of their ability to act as stem elements.
dcterms:title
Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors
skos:prefLabel
Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors Plasticity of Calcium Signaling Cascades in Human Embryonic Stem Cell-Derived Neural Precursors
skos:notation
RIV/00216208:11130/13:10209663!RIV14-GA0-11130___
n14:predkladatel
n15:orjk%3A11130
n3:aktivita
n13:P n13:I
n3:aktivity
I, P(GAP304/11/2373), P(GBP304/12/G069)
n3:cisloPeriodika
10
n3:dodaniDat
n9:2014
n3:domaciTvurceVysledku
n7:6738109
n3:druhVysledku
n8:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
96438
n3:idVysledku
RIV/00216208:11130/13:10209663
n3:jazykVysledku
n17:eng
n3:klicovaSlova
spinal neurons; nervous-system; progenitor cells; functional-properties; endoplasmic-reticulum; stem/progenitor cells; magnocellular neurons; neuronal differentiation; in-vitro; rat supraoptic nucleus
n3:klicoveSlovo
n5:endoplasmic-reticulum n5:functional-properties n5:nervous-system n5:magnocellular%20neurons n5:rat%20supraoptic%20nucleus n5:neuronal%20differentiation n5:progenitor%20cells n5:spinal%20neurons n5:in-vitro n5:stem%2Fprogenitor%20cells
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[9C4CA1C85835]
n3:nazevZdroje
Stem Cells and Development
n3:obor
n19:EB
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n18:GBP304%2F12%2FG069 n18:GAP304%2F11%2F2373
n3:rokUplatneniVysledku
n9:2013
n3:svazekPeriodika
22
n3:tvurceVysledku
Dayanithi, Govindan Verkhratsky, Alexei Romanyuk, Nataliya Syková, Eva Forostyak, Oksana
n3:wos
000318684900004
s:issn
1547-3287
s:numberOfPages
16
n20:doi
10.1089/scd.2012.0624
n21:organizacniJednotka
11130